Does Biological Sex Influence Insulin Sensitivity and Muscle Metabolism Following High-intensity Interval Exercise?
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 2
- 主要终点
- Insulin area under the curve
研究概览
简要总结
High-intensity interval exercise (HIIE) is a type of exercise that involves alternating periods of intense exercise with periods of rest. HIIE has been shown to improve many aspects of cardiovascular and metabolic health in a time-efficient manner (e.g., only 20 minutes per exercise session). An important health benefit of exercise is improved blood sugar control, which can help reduce the risk of metabolic diseases like type 2 diabetes. A single session of HIIE has been shown to improve blood sugar in males, but it is unknown if females achieve the same health benefit. It is also not fully understood how exercise improves blood sugar in males and females. Therefore, the purpose of this project is 1) to determine if a single session of HIIE improves blood sugar control in males and females, and 2) to evaluate if changes in skeletal muscle can explain the beneficial effects of HIIE on blood sugar.
详细描述
The primary purpose of this study is to determine if biological sex influences the effects of high-intensity interval exercise (HIIE) on insulin sensitivity and muscle mechanisms. The investigators will measure insulin sensitivity and muscle outcomes of participants on two separate occasions: 1) Following 30 minutes of sitting in the lab; and 2) Following a single session of HIIE on a stationary bike. Insulin sensitivity will be measured by taking blood samples after participants consume a sugary drink. Muscle outcomes will be measured by taking a small amount of muscle from the vastus lateralis (thigh) muscle. Groups of male and female participants will be recruited and tested using best practice guidelines for sex-based comparisons of exercise responses.
The study will advance knowledge regarding the potential for biological sex to influence the metabolic responses high-intensity exercise.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •18-35 yrs
- •VO2peak considered recreationally active as defined as 'fair' or 'good' (males: 41.6- 50.5ml/kg/min; females: 35-41.9 ml/kg/min) based on Canadian Society for Exercise Physiology (CSEP) normative fitness values.
- •BMI between 18-27 kg/m2
- •1-3 structured exercise sessions/week, and not training for any specific sport
- •Weight stable (within ± 2kg for at least 6 months)
- •Eumenorrheic (female only), defined as menstrual cycle lengths ≥ 21 days and ≤ 35 days resulting in 9 or more consecutive periods per year)
- •Non-smoker
排除标准
- •Diagnosed with cardiovascular or metabolic disease, hyper- or hypogonadism, and/or polycystic ovarian syndrome (PCOS)
- •The use of medication for managing blood glucose or lipid metabolism
- •Current use of oral contraceptives or use within the last 3 months
- •Irregular menstrual cycles (<21 days or >35 days)
- •Pregnant, lactating, or menopausal
- •Recreational smoking of any kind
- •Inability to perform the study exercise protocols or follow the pre-trial dietary or physical activity controls
- •Taking medications affecting substrate metabolism (corticosteroids or nSAIDs)
- •Actively engaging in a low-carbohydrate diet (e.g., ketogenic, Atkins)
结局指标
主要结局
Insulin area under the curve
时间窗: 3 hours
Insulin area under the curve measured during glucose tolerance test
次要结局
- Glucose area under the curve(3 hours)
- Insulin:glucose ratio(3 hours)
- Mean glucose concentration(3 hours)
- Peak insulin concentration(3 hours)
- Skeletal muscle glycogen content(immediately before and after exercise)
- Skeletal muscle insulin signaling(1hr into glucose tolerance test)
- Local muscle oxygenation(Pre-exercise, immediately post-exercise and 2 hrs post-exercise)
- Skeletal muscle contractile signaling protein content(immediately before and after exercise)
- Mean insulin concentration(3 hours)
- Postprandial glucose oxidation(3 hours)
- Muscle protein synthesis rates(3hr post-exercise)
- Peak glucose concentration(3 hours)
- Mitochondrial gene expression(Change from pre-exercise to 3hr post-exercise)
研究者
Jenna Gillen
Assistant Professor
University of Toronto