Effect of Passive Ultrasonic Irrigation on Bacteria That Persist After Root Canal Preparation

Not Applicable

Completed

• Conditions: Bacterial Infections
• Interventions: Procedure: Ultrasonic irrigation

• Registration Number: NCT05100433
• Lead Sponsor: University of Sao Paulo

Brief Summary

This study aims to evaluate the effectiveness of ultrasonic irrigation in improving root canal disinfection after chemomechanical procedures.

Detailed Description

Although chemomechanical procedures promote a drastic bacterial reduction, many root canals remain infected, which points to the need for complementary procedures to improve root canal disinfection. Thus, this clinical study aims to evaluate the effect of passive ultrasonic irrigation as a supplementary disinfection procedure after root canal preparation. Microbiological samples of the root canals of 30 single-rooted teeth with apical periodontitis will be taken at different stages of the endodontic treatment: before preparation (S1), after chemomechanical procedures (S2) and after passive ultrasonic irrigation (S3). The samples will be submitted to the quantitative polymerase chain reaction for bacterial quantification and to the reverse transcription quantitative polymerase chain reaction for bacterial activity analysis. Data will be analyzed using the Wilcoxon test for paired samples (p \< 0.05).

Study Timeline

• Study Start: 2018-03-05
• Study First Submitted: 2021-09-20
• Study First Submitted QC: 2021-10-18
• Study First Posted: 2021-10-29
• Primary Completion: 2022-11-25
• Study Completion: 2022-11-25
• Status Verified: 2023-11
• Last Update Submitted: 2023-11-28
• Last Update Posted: 2023-11-29

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 30

Inclusion Criteria

• Teeth with necrotic pulps and asymptomatic apical periodontitis

Exclusion Criteria

• patients who had received antibiotics during the previous 3 months or had any general disease,
• teeth that could not be properly isolated with rubber dam,
• non-restored teeth,
• periodontal pockets depths greater than 4 mm,
• previous endodontic treatment,
• open apex,
• crown/root fracture
• root resorption or calcifications.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Microbiological analysis of root canals after endodontic procedures	Ultrasonic irrigation	Bacterial levels and activity in root canal samples after chemomechanical procedures and ultrasonic irrigation.

Primary Outcome Measures

Name	Time	Method
Bacterial Activity	through study completion, an average of 3 years	The metabolic activity of total bacteria in root canal samples after ultrasonic irrigation

Secondary Outcome Measures

Name	Time	Method
Bacterial Levels	through study completion, an average of 3 years	Quantitative data of total bacteria determined by quantitative polymerase chain reaction after endodontic procedures

Trial Locations

Locations (1)

University of São Paulo; 🇧🇷 São Paulo, SP, Brazil