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Efficacy and Safety of a Half-dose Bolus of r-SAK Prior to Primary PCI in ST-elevation Myocardial Infarction

Phase 4
Recruiting
Conditions
ST Elevation Myocardial Infarction
Interventions
Registration Number
NCT05410925
Lead Sponsor
The First Affiliated Hospital with Nanjing Medical University
Brief Summary

As an effective treatment for acute ST-segment elevation myocardial infarction (STEMI), early reperfusion may reduce the infarct size and improve the prognosis of patients. However, it remains uncertain whether adjunctive thrombolytic therapy administered immediately prior to primary percutaneous coronary intervention (PCI) improves outcomes in patients undergoing the procedure within 120 minutes.

In this investigator-initiated, prospective, multi-center, randomized, double-blind, placebo-controlled trial, subjects meeting the inclusion/exclusion criteria should be randomly assigned 1:1 to the trial group (r-SAK) or the control group (placebo). The risk of major adverse cardiovascular events within 90 days will be observed.

Detailed Description

Acute myocardial infarction (AMI) is a serious and critical disease that causes acute coronary artery stenosis, spasm or occlusion due to the rupture or erosion of coronary artery plaque, resulting in myocardial ischemia and necrosis. Myocardial ischemia and necrosis can cause myocardial cell loss, ventricular remodeling and local inflammatory reaction, leading to decreased cardiac output or increased intracardiac pressure, and eventually progress to heart failure (HF), which seriously affects the prognosis of patients. The FAST-MI study found that 37.5% of AMI patients were complicated with HF, and the 1-year mortality of these patients was significantly increased. Early reperfusion treatment as an effective means of AMI treatment can promote myocardial reperfusion, save dying myocardium and reduce infarct area, which is of great significance to improve the clinical prognosis of patients.

At this stage, many primary hospitals do not have the conditions for emergency percutaneous coronary intervention (PCI). Transferring patients to PCI hospitals takes a lot of time, delaying the best time for early reperfusion treatment. In addition, the thrombus burden in the coronary artery increases with the prolongation of ischemia time. Stent implantation in the coronary artery with excessive thrombus burden is prone to slow blood flow or no reflow, resulting in the occurrence of major adverse cardiovascular events (MACE). In view of the above problems, guidelines suggest that if the estimated transit time is more than 120 minutes, thrombolytic therapy should be performed before transport; If the estimated transfer time is less than 120 minutes, it can be directly transferred to the PCI hospital.

However, it remains uncertain whether adjunctive thrombolytic therapy administered immediately prior to primary PCI improves outcomes in patients undergoing the procedure within 120 minutes ("facilitated PCI"). Multiple previous studies comparing facilitated PCI with primary PCI found facilitated PCI to be inferior in terms of clinical outcomes, while other studies based on reduced-dose thrombolysis confirmed the superiority of facilitated PCI in better patency of infarct-related artery (IRA). Recombinant staphylokinase (r-SAK), as the third-generation thrombolytic agent, may serve as the potential thrombolytic drug to contemporary facilitated PCI by virtue of its high fibrinolytic activity and fibrin selectivity.

Staphylokinase (SAK) is produced by Staphylococcus aureus and it is a protein containing 136 amino acid residues. Its ability for dissolving blood clots was first discovered in 1948. Studies have shown that SAK is not directly convert plasminogen (PLG) into plasminogen (PLi), but first combines with PLG in a 1:1 ratio to form a complex. The complex can lead to the exposure of PLG active site, from single chain to double chain PLi, resulting to form an active SAK-PLI complex, which subsequently activates PLG molecules. Then PLG transforms into PLi and further dissolve the thrombus.

R-SAK was developed in 1990 by Shanghai Institute of Plant and Biological Physiology. It is a gene recombinant drug prepared by molecular cloning of SAK gene in Escherichia coli. Its biological characteristics are very similar to natural SAK, and r-SAK is a highly fibrin-specific fibrinolysis agent. R-SAK is considered to be one of the most promising thrombolytic drugs due to its high thrombolysis activity (especially in platelet-rich arterial thrombosis), inactivation of system fibrinolysis, and few side effects. Clinical studies have shown that the efficacy of r-SAK in the treatment of AMI is better than urokinase, comparable to RT-PA, and it does not increase serious bleeding complications such as intracranial hemorrhage.

In terms of pharmacokinetics, r-SAK has a fast distribution and a long action time in human body. Half-lives of distribution term is 13.30±2.06min and elimination term is 67.94±21.39min when intravenous injection 10 mg r-SAK in 30min. A single bolus of r-SAK as early as possible during the first medical contact (such as prehospital care or primary hospitals or medical centers with conditional PCI) can maximize the time window for reperfusion therapy.

Achieving early reperfusion by means of facilitated PCI is consistent with the core of STEMI treatment, but the efficacy and safety of facilitated PCI are still controversial. OPTIMA-6, designed as shorter symptom onset to treatment time, a half-dose thrombolytic agent, and upstream use of the potent antiplatelet agent, will therefore evaluate the efficacy and safety of a half-dose bolus of r-SAK vs. placebo prior to primary PCI to inform clinical practice of contemporary facilitated PCI in patients with STEMI.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
2260
Inclusion Criteria
  1. Age 18-75 years, weight ≥45 kg

  2. Diagnosed as STEMI (meeting the following two conditions simultaneously)

    1. Ischemic chest pain lasts ≥ 30 minutes
    2. ECG indicates that ST-segment elevation of two or more contiguous precordial leads ≥ 0.1 mV, or ST-segment elevation of two or more contiguous precordial leads ≥ 0.2 mV
  3. Time from onset of persistent chest pain to randomization ≤12 hours

  4. Primary PCI expected to be performed ≥30 minutes, and ≤120 minutes

Exclusion Criteria
  1. Cardiogenic shock
  2. Active bleeding or known at high risk of bleeding (including grade Ⅲ or Ⅳ retinopathy or retinal gastrointestinal or urinary tract hemorrhage within the past 1 month)
  3. Ischemic stroke or TIA in the past 6 months
  4. History of hemorrhagic stroke
  5. Known intracranial aneurysm
  6. Severe trauma, surgery or head injury within 1 month
  7. Suspected aortic dissection or infective endocarditis
  8. Puncture with difficult hemostasis by compression within 1 month (e.g., visceral biopsy, compartment puncture)
  9. Currently taking anticoagulants
  10. Poorly controlled hypertension ( ≥180/110 mmHg)
  11. Severe hepatic or renal impairment indicated by the consultation or previous history (glutamic-pyruvic transaminase or glutamic oxalacetic transaminase >3 times upper limit of normal value; eGFR <15 ml/min/1.73m^2, calculated based on CKD-EPI)
  12. Known allergy to r-SAK
  13. Pregnancy, lactation, or planning for pregnancy
  14. History of chronic total occlusion, myocardial infarction or CABG
  15. Having taken antiplatelet drugs other than aspirin and ticagrelor, such as clopidogrel, prasugrel or cilostazol after the symptom onset
  16. Patients with other conditions that made them unsuitable to be recruited at the discretion of the investigators

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
placebo groupPlacebointravenous injection of placebo in 3 min
r-SAK groupRecombinant staphylokinaseintravenous injection of single-bolus 5 mg r-SAK in 3 min
Primary Outcome Measures
NameTimeMethod
MACEWithin 90 days

Defined as a composite of all-cause death, reinfarction, unplanned target vessel revascularization, heart failure or cardiogenic shock, major ventricular arrhythmia

Secondary Outcome Measures
NameTimeMethod
LVEF assessed by echocardiogramDay 360±7

LVEF assessed by echocardiogram

NT-proBNPDay 360±7

The level of NT-proBNP

The percentage of TIMI flow grade 3 prior to PCIImmediately prior to PCI

The percentage of TIMI flow grade 3 prior to PCI

The percentage of TIMI flow grade 2 and 3 prior to PCIImmediately prior to PCI

The percentage of thrombolysis in myocardial infarction (TIMI) flow grade 2 and 3 prior to PCI

The percentage of TIMI flow grade 2 and 3 after PCIImmediately after PCI

The percentage of TIMI flow grade 2 and 3 after PCI

Each of the following cardiac and cerebrovascular eventsWithin 360 days

Including all-cause death, cardiovascular death, reinfarction, ischemic stroke, unplanned target vessel revascularization, heart failure or cardiogenic shock, major ventricular arrhythmia, cardiogenic rehospitalization, ventricular septal rupture, papillary muscle rupture, cardiac rupture, ventricular aneurysm

The percentage of TIMI flow grade 3 after PCIImmediately after PCI

The percentage of TIMI flow grade 3 after PCI

MACEWithin 360 days

Defined as a composite of all-cause death, reinfarction, unplanned target vessel revascularization, heart failure or cardiogenic shock, major ventricular arrhythmia

CMR indexesDay 5

Including infarct size, left ventricular ejection fraction (LVEF), microvascular obstruction (MVO) and intramuscular hemorrhage (IMH), assessed by cardiac magnetic resonance (CMR)

Trial Locations

Locations (61)

Huai'an Second People's Hospital

🇨🇳

Huai'an, China

The First People's Hospital of Lianyungang

🇨🇳

Lianyungang, China

The Fourth Affiliated Hospital of Nanjing Medical University

🇨🇳

Nanjing, China

Renji Hospital affiliated to Shanghai Jiaotong University

🇨🇳

Shanghai, China

Taizhou People's Hospital

🇨🇳

Taizhou, China

Affiliated Hospital of Jiangnan University

🇨🇳

Wuxi, China

Wuxi Second People's Hospital

🇨🇳

Wuxi, China

Xuzhou Central Hospital

🇨🇳

Xuzhou, China

Yancheng No.1 People's Hospital

🇨🇳

Yancheng, China

Zhenjiang First People's Hospital

🇨🇳

Zhenjiang, China

The First Affiliated Hospital of Nanjing Medical University

🇨🇳

Nanjing, Jiangsu, China

The Second Affiliated Hospital of Zhejiang University Medical College

🇨🇳

Hangzhou, China

Huai'an First People's Hospital

🇨🇳

Huai'an, China

Changzhou Second People's Hospital

🇨🇳

Changzhou, China

Changzhou Wujin People's Hospital

🇨🇳

Changzhou, China

The First People's Hospital of Changzhou

🇨🇳

Changzhou, China

Daqing Oilfield General Hospital

🇨🇳

Daqing, China

Dongguan People's Hospital

🇨🇳

Dongguan, China

Fengcheng People's Hospital

🇨🇳

Fengcheng, China

General Hospital of Southern Theatre Command

🇨🇳

Guangzhou, China

The Second Affiliated Hospital of Hainan Medical University

🇨🇳

Hainan, China

The Affiliated Hospital of Hangzhou Normal University

🇨🇳

Hangzhou, China

The First Affiliated Hospital of Anhui Medical University

🇨🇳

Hefei, China

Donghai Country People's Hospital

🇨🇳

Lianyungang, China

The Second People's Hospital of Lianyungang

🇨🇳

Lianyungang, China

Liyang Hospital of Jiangsu Province Hospital

🇨🇳

Liyang, China

The First Affiliated Hospital of Nanchang University

🇨🇳

Nanchang, China

Nanjing Tongren Hospital

🇨🇳

Nanjing, China

Sir Run Run Hospital Nanjing Medical University

🇨🇳

Nanjing, China

Nanjing Qixia District Hospital

🇨🇳

Nanning, China

Affiliated Hospital of Nantong University

🇨🇳

Nantong, China

Nantong First People's Hospital

🇨🇳

Nantong, China

The People's Hospital of Zhalaite

🇨🇳

Neimeng, China

Qingdao Municipal Hospital

🇨🇳

Qingdao, China

Qilu Hospital of Shandong University

🇨🇳

Shandong, China

Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

🇨🇳

Shanghai, China

The People's Hospital of Liaoning Province

🇨🇳

Shenyang, China

Shenzhen People's Hospital

🇨🇳

Shenzhen, China

Suining County People's Hospital

🇨🇳

Suining, China

Nanjing Drum Tower Hospital Group Suqian Hospital

🇨🇳

Suqian, China

Suqian First Hospital

🇨🇳

Suqian, China

Suzhou Dushu Lake Hospital

🇨🇳

Suzhou, China

Suzhou Municipal Hospital of Anhui Province

🇨🇳

Suzhou, China

Suzhou Municipal Hospital

🇨🇳

Suzhou, China

The First Affiliated Hospital of Soochow University

🇨🇳

Suzhou, China

Wanbei Coal-Electricity Group General Hospital

🇨🇳

Suzhou, China

Taihe County Traditional Chinese Medicine Hospital

🇨🇳

Taihe, China

Taishan People&#39;s Hospital

🇨🇳

Taishan, China

The First Affiliated Hospital of Wenzhou Medical University

🇨🇳

Wenzhou, China

The Second Affiliated Hospital of Wenzhou Medical University

🇨🇳

Wenzhou, China

Wuxi People's Hospital

🇨🇳

Wuxi, China

The Affiliated Hospital of Xuzhou Medical University

🇨🇳

Xuzhou, China

The People's Hospital of Jiawang District of Xuzhou City

🇨🇳

Xuzhou, China

Chongqing Hospital of Jiangsu Province Hospital

🇨🇳

Chongqing, China

Affiliated Hospital of Yangzhou University

🇨🇳

Yangzhou, China

Subei People's Hospital of Jiangsu province

🇨🇳

Yangzhou, China

Fuwai Central China Cardiovascular Hospital

🇨🇳

Zhengzhou, China

The 7th People's Hospital of Zhengzhou

🇨🇳

Zhengzhou, China

The First Affiliated Hospital of Zhengzhou University

🇨🇳

Zhengzhou, China

Affiliated Hospital of Jiangsu University

🇨🇳

Zhenjiang, China

The second Affiliated Hospital of Dalian Medical University

🇨🇳

Dalian, China

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