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Effects of Light on the Choroid and Pupil

Not Applicable
Completed
Conditions
Emmetropia
Myopia
Interventions
Other: White Light therapy
Other: Darkness
Other: Red light therapy
Other: Blue light therapy
Registration Number
NCT05852782
Lead Sponsor
University of Houston
Brief Summary

Monochromatic light creates longitudinal chromatic aberration, with short wavelength blue light forming a focal point in front of the retina and long wavelength red light forming a focal point behind the retina. The investigators hypothesize that such chromatic aberrations, induced by exposure to red or blue LED lights, will cause the choroid behind the retina to respond to bring the image into focus by modulating its thickness, either thickening in the case of blue light or thinning in the case of red light. The magnitude and direction of this response is difficult to predict as previous studies have shown opposite findings in non-human primates and rodents. Furthermore, the investigators hypothesize that exposure to red or blue light will induce changes in how the pupil responds to light, because the cells in the eye that are involved in pupil control are most sensitive to blue light.

Detailed Description

Ambient light exposure is associated with choroid thickness and eye growth. The spectral composition of light, which has been shown to play a role in eye growth, can differentially regulate changes in choroid thickness by forming a focal point either in front of the retina in the case of short wavelength blue light or behind the retina in the case of long wavelength red light. In addition, melatonin suppression and the pupil light response, specifically, that driven by the intrinsically photosensitive retinal ganglion cells (ipRGCs), are most sensitive to short wavelength blue light. Therefore, the role of the wavelength of light on eye growth may involve ipRGC activity. As such, it is relevant to determine whether exposure to monochromatic light will alter choroidal thickness, ipRGC activity and melatonin levels.

The purpose of this study is to compare the short-term changes in choroid thickness, melatonin concentration, and the ipRGC-driven pupillary light response following one hour of light therapy with either short wavelength blue light or long wavelength red light. It has previously been shown that a one week period of daily morning light therapy produces a short term increase in choroid thickness throughout the day. Therefore, the investigators hypothesize that one hour of morning light therapy with short wavelength blue light will result in different effects in the changes in choroid thickness, melatonin levels, and the ipRGC-driven pupillary light response compared to one hour of morning light therapy with long wavelength red light.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
20
Inclusion Criteria
  • ages 18-64
  • must be able to comply with the experimental protocol, including collecting saliva and being present for the experiment in the lab on four separate occasions.
  • must be willing to abstain from the use of sleep aids 12 hours prior to and for the duration of the study.
  • must also be willing to abstain from alcohol and caffeinated beverages (coffee, espresso, energy drinks) or foods enriched with caffeine, such as power bars, the morning of and during each of the four experimental sessions.
Exclusion Criteria
  • age < 18 or >64 years old
  • not willing to collect a saliva sample or be present in the lab on four separate occasions.
  • ocular disease that may affect retinal light levels and function (e.g. glaucoma, significant cataract, etc.)
  • use of prescription or over-the-counter medications known to affect sleep and cortisol levels.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Light exposure broadbandWhite Light therapyParticipants will be exposed to indoor levels of light that appear colorless for one hour
Light exposure darknessDarknessParticipants will be exposed to darkness for one hour
Light exposure long wavelengthRed light therapyParticipants will be exposed to indoor levels of light that appear red for one hour
Light exposure short wavelengthBlue light therapyParticipants will be exposed to indoor levels of light that appear blue for one hour
Primary Outcome Measures
NameTimeMethod
Choroidal Thicknessone hour

The choroid will be imaged with OCT to determine choroidal thickness

Pupil responseone hour

The pupil response will be measured before and after one hour to see if exposure to light affects the diameter

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

University of Houston College of Optometry

🇺🇸

Houston, Texas, United States

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