A Comparative Study of Monozygotic and Dizygotic Twins: Evaluating the Effects of a 12-Week Balanced Diet Intervention on Body Composition and Metabolic Parameters.
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- Change in Body Fat Percentage (%)
研究概览
简要总结
Twin study in Lahore will assess genetic vs. environmental effects on diet and metabolism. Over 12 weeks, monozygotic and dizygotic twins will follow a WHO-aligned balanced diet. Body composition, metabolic markers, and adherence will be measured. Monozygotic twins are expected to show greater similarity in response, clarifying personalized nutrition strategies in Pakistan.
详细描述
Background: Twin studies help separate genetic and environmental influences on diet and metabolic health. With rising obesity and metabolic disorders in Pakistan, there is no twin-based dietary intervention data. Filling this gap is essential for developing effective, evidence-based personalized nutrition strategies.
Hypothesis
The null hypothesis states that there will be no difference in dietary response or within-pair similarity between monozygotic and dizygotic twins. The alternative hypothesis suggests that monozygotic twins will show greater similarity in diet response and that the balanced diet will significantly improve metabolic outcomes.
Methodology: A 12-week randomized controlled trial will recruit Monozygotic and Dizygotic twins aged 18-60 years in Lahore, Pakistan. Participants of interventional group will follow a WHO-aligned balanced diet. Body composition, metabolic markers, and dietary adherence will be assessed at baseline and week 12.
Statistical Design: Analyses will be conducted in SPSS using paired t-tests for within-pair comparisons and independent t-tests for Monozygotic vs. Dizygotic differences. Effect sizes will be calculated, with significance set at p < 0.05.
Expected Outcomes: The study is expected to show that the balanced diet improves metabolic and body composition outcomes, with monozygotic twins displaying more similar responses than dizygotic twins. These findings will clarify genetic vs. environmental effects and support more targeted nutrition recommendations in Pakistan.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Male or female twins aged 18-60 years.
- •Confirmed zygosity (MZ or DZ) based on a validated twin zygosity questionnaire.
- •Both twins willing to participate for the entire intervention period.
- •Free from chronic diseases requiring specialized diets (e.g., type 1 diabetes, celiac disease).
- •Not currently enrolled in another dietary or lifestyle intervention study.
排除标准
- •Pregnant or lactating women.
- •Use of lipid-lowering, glucose-lowering, or weight-loss medications in the past 3 months.
- •Major gastrointestinal disorders or conditions affecting nutrient absorption.
- •Unwillingness to follow individualized dietary counseling or complete study assessments.
研究组 & 干预措施
Habitual Diet
Control group will remain on habitual Diet.
Balanced Diet
12-week culturally adapted WHO-aligned balanced diet emphasizing whole grains, lean proteins, healthy fats, fruits, and vegetables, individualized based on basal metabolic rate (BMR) and physical activity levels will be provided to interventional arm.
干预措施: Balanced Diet (Other)
结局指标
主要结局
Change in Body Fat Percentage (%)
时间窗: Baseline and after 12-Week of Intervention.
Change in total body fat percentage measured using the InBody analyzer to assess the effect of a 12-week balanced diet intervention.
次要结局
- Change in Fasting Plasma Glucose (mg/dL)(Baseline and after 12 weeks)
- Change in Lipid Profile (mg/dL)(Baseline and after 12 weeks)
- To compare within-pair similarity of Dietary Response(Baseline and after 12-Weeks of intervention)
研究者
Qaisar Raza
Assistant Professor
University of Veterinary and Animal Sciences, Lahore - Pakistan