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Dietary Supplement on the Intestinal Microbiota in Patients with Colon Cancer

Not Applicable
Completed
Conditions
Colon Cancer
Interventions
Dietary Supplement: Experimental Treatment, DCOOP Product, Hydroxytyrosol extract
Dietary Supplement: Experimental Treatment,Indukern product, Curcumin and selenium extract
Other: Control Treatment
Registration Number
NCT05472753
Lead Sponsor
Fundación Pública Andaluza para la Investigación de Málaga en Biomedicina y Salud
Brief Summary

Effect of a dietary supplement with antioxidant and anti-inflamatory properties on the intestinal microbiota in patients with colon cancer. Ramdonized placebo controlled clinical trial. Teratrophic study

Detailed Description

Introduction:

The alteration in the microbiota plays a fundamental role in the promotion and progression of colon cancer due to various pathways such as inflammation and oxidative stress. The use of substances with anti-inflammatory and antioxidant effect could be useful for the treatment of this disease.

Methodology:

Prospective randomized clinical trial, with three parallel groups and double blind. Patients with stage II or III colon neoplasia who are going to receive post-surgical chemotherapy will be included. Patients will be randomized to one of the following groups: group 1 (25 patients): product with hydroxytyrosol extract; group 2 (25 patients): product with curcumin and selenium extract. Group 3 (25 patients): placebo. Before starting chemotherapy, stool and blood samples will be taken, and gastrointestinal symptoms, quality of life, symptoms of anxiety-depression and evaluation of nutritional status will be assessed. When starting chemotherapy, they will start with a daily intake of the assigned dietary supplement. At 3 months ± 2 weeks after starting chemotherapy (at least 2 weeks must have passed since the last chemotherapy of the fourth cycle), the same assessment will be made as in the initial visit, in addition to recording adherence to the intervention dietary supplement and new health problems that have appeared since the previous visit.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
75
Inclusion Criteria
  • Diagnosis of stage II or III colo neoplasia
  • Indication of adjuvant chemotherapy according to the Protocols for the diagnosis and treatment of cancer of the Intercenter Clinical Management Unit
  • Sign the informed consent
Exclusion Criteria
  • Systemic autoimmune diseases (systemic lupus erythematosus, antiphospholipid syndrome, Sjögren's syndrome, progressive systemic sclerosis -scleroderma-, idiopathic inflammatory myopathies -myositis-, vasculitis, Behçet's disease, relapsing polychondritis, etc.)
  • Mellitus diabetes type 1
  • Previous gastrointestinal resections, except for appendectomy or surgery required to treat colon neoplasia
  • Chronic intestinal pathologies (inflammatory bowel disease, celiac disease, lymphangiectasias)
  • Continued consumption of probiotics, with the exception of dairy products or other natural fermented foods
  • Chronic and continued use of NSAIDs or corticosteroids
  • Allergy to any component of the product under investigation
  • Pregnancy
  • Mean consumption of > 3 UBE of alcohol per day
  • Previous or concomitant neoplasia, unless curative treatment was received and ≥5 years have passed free of disease
  • ECOG scale greater than or equal to 3 at the start of the clinical trial
  • Grade 3-4 neuropathy that limits the use of oxaliplatin.
  • History of familial adenomatous polyposis mediated by the APC gene or by Lynch syndrome (mutations MLH1, MSH2, PMS2, MSH6).
  • Patients with partial or complete deficiency of the enzyme dihydropyrimidine dehydrogenase (DPD), which causes poor metabolism and the use of fluoropyrimidines is contraindicated.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Group 1 (25 patients)Experimental Treatment, DCOOP Product, Hydroxytyrosol extractA product of the company DCOOP, with hydroxytyrosol extract
Group 2 (25 patients)Experimental Treatment,Indukern product, Curcumin and selenium extractA product of the company Indukern, with extract of curcumin and selenium
Group 3 (25 patients)Control TreatmentPlacebo
Primary Outcome Measures
NameTimeMethod
Change in the alpha diversity index (Shannon)From baseline to 3 months about 2 weeks after starting chemotherapy

This diversity index is a quantitative indicator of the number of different bacteria that are present in a stool sample, taking into account the uniformity in the distribution of these bacteria in these species. Diversity index value increases both when the number of species increases and when evenness increases. The Shannon index is a well-known diversity index used in microecological studies. The higher the Shannon index value, the higher the community diversity. It is calculated as: H = -Σpi \* ln(pi), where "H" is the Shannon Diversity Index. "Σ" is a Greek symbol that means "sum". "ln" is natural log. "pi" is the proportion of the entire community made up of species i. The minimum value the Shannon diversity index can take is 0. Such a number would tell us that there is no diversity - only one species is found in that habitat. There is no upper limit to the index.

Secondary Outcome Measures
NameTimeMethod
Change in heightFrom baseline to 3 months about 2 weeks after starting chemotherapy

Height in m

Change in weightFrom baseline to 3 months about 2 weeks after starting chemotherapy

Weight in kg

BMI (body mass index) changesFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured by body composition analysis

Mediterranean diet adherence questionnaireFrom baseline to 3 months about 2 weeks after starting chemotherapy

to evaluate adherence to a Mediterranean diet pattern. It consists of 14 items in which different components of the Mediterranean diet are evaluated (number of pieces of fruit consumed per day, number of portions of legumes consumed per week...). Each item is scored as 0 or 1. A total score of \<9 indicates poor adherence, while a score of ≥9 indicates good adherence.

Mean dominant arm circumferenceFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in cm

Maximum and mean value of 3 measurements in dominant hand dynamometryFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in kg

Calf circumference in the dominant legFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in cm

Transverse perimeter of the quadriceps rectus muscle in the dominant thighFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in cm

Mean value of 3 measurements of the anteroposterior diameter of abdominal visceral adipose tissueFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in cm

Extracellular water in bioimpedanciometryFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in kg

Maximum and mean value of 3 measurements in non dominant hand dynamometrydynamometryFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in kg

Anteroposterior diameter of the rectus femoris of the quadriceps in the dominant thighFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in cm

Transverse diameter of the rectus femoris of the quadriceps in the dominant thighFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in cm

Cross-sectional area of the rectus femoris quadriceps in the dominant thighFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in cm²

Mean value of 3 measurements of the anteroposterior diameter of abdominal subcutaneous adipose tissueFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in cm

Fat mass in bioimpedanciometryFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in kg

Lean mass in bioimpedanciometryFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in kg

Phase angle 50 kHz in bioimpedance measurementFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in º

Percentage of weight lost in the last 12 monthsFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in %.

Mean corpuscular volume in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in fL

Creatinine in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in g/dl

Chlorine in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in mEq/l

Calcium in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in mg/dl

Total cell mass in bioimpedance measurementFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in kg

Percentage of weight lost in the last 6 monthsFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in %.

Glucose in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in mg/dl

Phosphorus in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in mg/dl

Magnesium in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in mg/dl

Appendicular muscle mass in bioimpedanciometryFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in kg

Hemoglobin in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in g/dl

Potassium in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in mEq/l

Iron in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in mcg/dl

Blood cholesterolFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in mg/dl

LDL cholesterol in the bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in mg/dl

Bilirubin in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in mg/dl

Albumin in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in g/dl

25OH vitamin D in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in ng/ml

Normalized prothrombin timeFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in INR

Assess the effect of supplementation on quality of life: "EORTC QLQ-CR29" test.From baseline to 3 months about 2 weeks after starting chemotherapy

It is a supplemental questionnaire module to be used together with the QLQ-C30. The QLQ-CR29 incorporates 4 multi-item and 19 single-item scales. A variety of common symptoms and problems are assessed in colorectal cancer patients. The scoring approach is identical in principle to that of the scales or isolated items of functionality and symptoms of the QLQ-C30. All scales and single-item measures have a score range of 0 to 100. A high score on the functionality scale and the isolated items of functionality represents a high level of functionality, while a high score for the scales and isolated items represents a high level of functionality. of symptoms represents a high level of symptomatology or problems.

Leukocytes in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in x10\^9/l

Lymphocytes in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in x10\^9/l

Platelets in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in x10\^9/l

Sodium in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in mEq/l

HDL cholesterol in the bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in mg/dl

GGT in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in U/L

Degree in "ECOG Performance Status Scale"From baseline to 3 months about 2 weeks after starting chemotherapy

This scale was developed by the Eastern Cooperative Oncology Group (ECOG), currently the ECOG-ACRIN Cancer Research Group. It was published in 1982. The scale describes the level of functioning of a patient in terms of her ability to take care of herself, perform daily physical activity and physical capacity (walk, work...). It was devised to use standard criteria between different centers to measure how the disease affects a patient's daily living abilities. The scale has a score from 0 to 5 in whole numbers. Lower score indicates better functional capacity. Score 0 refers to fully active patients, capable of carrying out all activities as before the onset of the disease. A score of 5 indicates the death of the patient.

Blood triglyceridesFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in in mg/dl

ALT in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in U/L

Alkaline phosphatase in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in U/L

Total protein in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in g/dl

Ferritin in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in ng/ml

Prealbumin in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in mg/dl

Assess the effect of supplementation on quality of life: "EORTC QLQ-30" test.From baseline to 3 months about 2 weeks after starting chemotherapy

The "EORTC quality of life questionnaire (QLQ)" is an integrated system to assess the quality of life of cancer patients participating in international clinical trials. The main questionnaire is the "QLQ-C30". Version 3.0 is currently the standard version. The QLQ-C30 is made up of multiple-item and single-item scales. Five functionality scales, three symptom scales, a global health status/quality of life scale, and six individual items are included. Each of the multiple item scales includes a different set of items; no item appears on more than one scale. All single-item scales and measures range in score from 0 to 100. A high scale score represents a higher level of response. Thus, a high score for a functionality scale represents a high/healthy level of functionality, a high score for global health status/quality of life represents a high quality of life, but a high score for a scale or isolated items of symptoms represents a high level of symptoms/problems.

C-reactive protein in bloodFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in mg/dl

Assess the effect of supplementation on symptoms of depression-anxietyFrom baseline to 3 months about 2 weeks after starting chemotherapy

Hospital Anxiety and Depression Scale (HADS). Its objective is to detect depressive and anxious disorders in non-psychiatric hospital services, avoiding overlapping with symptoms due to physical illness, without taking into account the physical aspects that may accompany anxiety/depression, focusing only on emotional ones. It is a self-administered scale with 14 items divided into 2 subscales (anxiety and depression) whose maximum score is 21 points for each of them. Based on the score obtained, patients can be classified as normal (\<7), doubtful (between 8 and 10) and potential clinical case (≥11). The score is referred to the last week.

Assess gastrointestinal tolerance to supplementation and chemotherapyFrom baseline to 3 months about 2 weeks after starting chemotherapy

Gastrointestinal symptoms questionnaire: an own questionnaire created for this study will be carried out in which the presence of nausea, vomiting, diarrhoea, constipation, acid reflux, early satiety, abdominal distension and abdominal pain will be evaluated. Each variable is evaluated on a scale with 4 possible responses: absent, mild, moderate, severe.

Change in serum C-reactive proteinFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in mg/l

Change in serum Il-6From baseline to 3 months about 2 weeks after starting chemotherapy

Measured in pg/ml

Change in serum TNF-alphaFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in pg/ml

Change in stool calprotectinFrom baseline to 3 months about 2 weeks after starting chemotherapy

Measured in mcg/g

Trial Locations

Locations (1)

Hospital Regional Universitario de Málaga

🇪🇸

Málaga, Spain

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