The influence of a single bout of low compared to moderate-intensity exercise on white blood cell telomerase activity in young and older adults: The Telomerase Activity after LOw and Moderate Intensity Exercise (TALOMIE) trial

Not Applicable

Recruiting

• Conditions: Cardiovascular disease; Type 2 diabetes; Dementia; Cardiovascular - Other cardiovascular diseases; Metabolic and Endocrine - Diabetes; Neurological - Dementias

• Registration Number: ACTRN12621001546842
• Lead Sponsor: niversity of Southern Queensland

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2021-11-12
• Last Update Posted: 8 January 2024

Recruitment & Eligibility

• Status: Recruiting
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

1. Aged between 18-45 years; or 65-80 years
2. Free from cardiovascular disease.
3. Body mass index (BMI) <35
4. Free from any signs or symptoms of fever or upper respiratory tract infections.
5. Free from any musculoskeletal injuries or other conditions that would restrict the individual's ability to perform exercise safely.
6. Non-smoker

Exclusion Criteria

1. Previous diagnosis of myocardial infarction, stroke or angina.
2. Vaccination for SARS-COV-2 within the past 1 month or scheduled SARS-COV-2 booster vaccine within the timeline of the trial.
3. Signs and symptoms of fever or respiratory infection within the past 2 weeks.
4. Body mass index (BMI) >35
5. Osteoporosis - bone mineral density (t-score) < -2.5
6. Severe depression, anxiety and/or stress according to the DASS; or diagnosis of psychotic disorder.
7. Currently taking medication for cancer or immunological disorder.
8. A musculoskeletal injury that inhibits the individual's ability to perform exercise safely.
9. Evidence of substance abuse (e.g. pharmaceuticals, alcohol or other narcotics)

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Peripheral blood mononuclear cell (PBMC) telomerase reverse transcriptase (TERT) gene expression will be quantified by quantitative PCR. Gene expression relative to the control gene, GAPDH, will be used in statistical analyses.[ Basal (before each exercise bout), immediately after and 1 hour after the completion of each exercise bout (primary timepoint).];PBMC telomerase activity quantified by TRAPeze RT Telomerase Detection Kit.[ Basal (before each exercise bout), immediately after (primary timepoint) and 1 hour after the completion of each exercise bout.]