DRKS00030257CompletedNot Applicable
The structural integrity of mitochondrial DNA reveals new molecular perspectives for early diagnosis of colorectal cancer - mtDNA-KRK
Medizinischen Hochschule Brandenburg (MHB) Theodor Fontane, Dezernat für Wissenschaft und Forschung0 sites81 target enrollmentStarted: September 29, 2022Last updated:
Conditions
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Enrollment
- 81
Study Overview
Brief Summary
Results: Total cfDNA levels were significantly higher in CRC group compared to healthy control and increased with tumour stage. Long nuclear fragment levels were significantly lower in CRC patients. The integrity indices of nuclear cfDNA decreased from controls to patients with highly malignant tumor. Mitochondrial cfDNA fragment quantities were strongly reduced in early and late stages of tumor patients. Predictive models based on different analysis and predictor sets showed comparable classification performance. Conclusion: Increased blood cfDNA concentration in late UICC stages inversely correlate with nuclear cfDNA integrity index and suggest that necrotic degradation is not a major cause for higher total cfDNA quantity.
Study Design
- Study Type
- Observational
Eligibility Criteria
- Ages
- 18 Years to one (—)
- Sex
- All
Inclusion Criteria
- •1. Healthy subjects (must be available for purchase for research purposes (at Central Biobank GmbH) between the ages of 18 and 90
- •2. All male and female colorectal cancer patients (> 18 years) are included with the ability to understand and question the relevant patient information with a diagnosis of colorectal cancer who are referred for surgery
- •3. Patient's willingness to provide blood and tissue samples for scientific analysis as part of the preliminary study
- •4. A written declaration of consent (for healthy people and patients) must be available
Exclusion Criteria
- •1. Healthy subjects under 18 years of age
- •2. Colorectal cancer patients with manifest coronary heart disease, chronic heart failure and peripheral arterial disease
- •3. Colorectal cancer patients with other tumor diseases
- •4. Colon cancer patients with advanced renal failure
- •5. Colon cancer patients with chronic inflammatory diseases
- •6. Healthy subjects and patients who do not meet the inclusion criteria or who their Withdraw consent to participate in the study
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