Effects of Concentric and Eccentric Exercise Regimens on Bioenergetic Efficiency of Lymphocytes in Sedentary Males
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 33
- 试验地点
- 2
- 主要终点
- Mitochondrial function of lymphocyte
研究概览
简要总结
Eccentric cycling exercise (ECE) features lower metabolic demand and higher improvement of muscle strength compared to traditional concentric cycling exercise (CCE). Mitochondria can regulate energy metabolism and adaptive immune quality in T lymphocytes. However, the effects of ECE on mitochondrial functions in T-lymphocytes have not yet been established.
Method: A total of 33 healthy sedentary males were randomized and divided into ECE (n=11), CCE (n=11), and control groups (n=11). These subjects progressively performed CCE or ECE from 60% to 80% maximal workload on a bicycle ergometer for 40 min/day, 5 days/week for 6 weeks. A graded exercise testing and an isokinetic strength test were conducted to evaluate cardiopulmonary fitness and muscle strength, respectively. Phenotypes and mitochondrial respiratory capacity in T lymphocyte were analyzed using flow cytometry and high-resolution respirometer, respectively.
详细描述
Eccentric exercise training (EET) increases physical performance while having lower metabolic demand than concentric exercise training (CET).
Mitochondria of lymphocytes are essential for cell proliferation, death, and differentiation, and play a critical role in establishing lymphocyte phenotypes and their functions.
Whether EET influences lymphocyte bioenergetic efficiency remains unclear. The study was to investigate the effects of ECE and CCE regimens on adaptive immune functions and mitochondrial bioenergetics of T lymphocytes in sedentary males.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Outcomes Assessor)
入排标准
- 年龄范围
- 20 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Sedentary lifestyle.
- •Nonsmokers, nonusers of medications/vitamins.
- •No any cardiopulmonary/hematological risks.
排除标准
- •Have regular exercise habits (i.e., exercise frequency once per week, duration >20 min).
- •Have retabolic or musculoskeletal disease.
结局指标
主要结局
Mitochondrial function of lymphocyte
时间窗: 12 weeks
Evaluate bioenergetic health index (BHI) of mitochondria in lymphocyte by using a high resolution respirometer.
次要结局
- Cardiopulmonary fitness(12 weeks)
研究者
Jong-Shyan Wang
Principal Investigator
Chang Gung Memorial Hospital