Mitochondrial Energy Metabolism in Obese Women

Not Applicable

Completed

• Conditions: Metabolism Disorder; Mitochondrial Alteration; Physical Activity; Obesity
• Interventions: Other: Physical Training

• Registration Number: NCT03119350
• Lead Sponsor: University of Sao Paulo

Brief Summary

Considering that the failure of the treatment of obesity is justified by the multifactorial pathophysiology of this morbidity, the present project has the following hypotheses:

1. The occurrence of obesity is due to the derange,ent of mitochondrial energy metabolism ;

2. The unbalance is therapeutically modified through physical training ;

3. Obesity courses with the break-down in energy metabolism mitochondrial disease associated with systemic inflammatory characteristics that can be corrected through a combined long-term physical training program.

This study have as objective : to analyse changes in mitochondrial function, inflammatory profile, oxidative stress and energy metabolism caused by concurrent physical training in obese women.

Detailed Description

Specific objectives:

Body composition by deuterium oxide; Metabolic rate of resting and oxidation of substrates by indirect calorimetry; Proinflammatory cytokines Anti-inflammatory cytokines Oxidative Stress: Malondialdehyde, Superoxide Dismutase, Glutathione-Peroxidase; Fatty acids: ceramide and palmitate; Mitochondrial respiration and citrate synthase enzyme; Quantify and qualify: mitochondrial number, endoplasmic reticulum structure, adipose cell size; Gene expression, quantify by microscopy and analyze the protein by western blot.

The study began with 20 women, however, there was withdrawal of 6, ending with 14 women.

Basic Information
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Recruitment & Eligibility
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Study & Design
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Trial Locations

Study Timeline

• Study Start: 2016-04-01
• Primary Completion: 2016-07-01
• Study Completion: 2016-09-15
• Study First Submitted: 2017-04-10
• Study First Submitted QC: 2017-04-13
• Study First Posted: 2017-04-18
• Status Verified: 2019-04
• Last Update Submitted: 2019-04-27
• Last Update Posted: 2019-04-30

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 14

Inclusion Criteria

• This study included women with obesity (BMI of 30 to 40 kg / m²), sedentary, with no associated comorbidity, convenience sample

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Exclusion Criteria

• Women who have undergone bariatric surgery, menopause, cancer or any metabolic disease, smokers, alcoholics, who are in use of drugs that act directly on the metabolism and that have medical impediment to the practice of physical exercise.

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Physical Training	Physical Training	There was concurrent physical training intervention: strength and aerobic exercises in the same session. Duration: 2 weeks of adaptation and learning to exercise, 8 weeks of physical training. Frequency: 3 times per week Duration: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate.

Primary Outcome Measures

Name	Time	Method
Changes inflammatory cytokines	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	Collected in lithium heparin tubes, they were centrifuged.
Changes Physical Performance	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	Based on the Shuttle Walking Test adaptation.
Changes Telomere length	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	peripheral blood in ethylenediaminetetraacetic acid tubes and genomic DNA was automatically extracted from Peripheral Blood Mononuclear Cell. The relative quantification of Telomere length was determined using the telomere to single copy gene ratio by Quantitative Polymerase Chain Reaction (qPCR).
Changes in fatty acids	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	Collected in lithium heparin tubes, they were centrifuged.
Changes in total cholesterol	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	Collected in lithium heparin tubes, they were centrifuged.
Changes in Determination of Lactate	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	Blood samples were collected by manual puncture of the earlobe in previously calibrated and heparinized capillary tubes, stored in eppendorf with sodium fluoride. Analyzed by electrochemical lactate analyser.
Changes Nitrogen Balance	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	Through the collection of urine of 24 hours the dosage of urinary nitrogen will be made by the chemiluminescence method for determination of protein nitrogen.
Changes Indirect calorimetry	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	With a gas analyzer (indirect calorimeter), we evaluated the metabolic rate and rest (REE) and oxidation of substrates (Lipids and carbohydrates).
Changes oxidative stress	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	Collected in lithium heparin tubes, they were centrifuged.
Changes Food intake	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	Food registry of 3 days, the quantification of the daily intake of nutrients will still be made using software.
Changes Body weight	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	Body weight was measured by digital balance before and after the intervention
Changes Body composition	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	The change in body composition through deuterium oxide was evaluated.
Changes White adipose tissue biopsy	Two times: (1) First day and (2) 10 weeks after adaptation and intervention	A subcutaneous tissue sample was collected for analysis of: mitochondrial respiration, citrate synthase enzyme, gene expression (UCP1, 2 and 3).

Trial Locations

Locations (1)

Camila Fernanda Cunha Brandão; 🇧🇷 Ribeirao Preto, SP, Brazil