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Comparison Between Early-onset and Late-onset Patients With Systemic Lupus Erythematosus.

Conditions
Systemic Lupus Erythematosus
Interventions
Diagnostic Test: CBC
Diagnostic Test: Antinuclear Antibody tests (ANA)
Diagnostic Test: Rest of ANA profile
Diagnostic Test: C3 and C4 complement level.
Diagnostic Test: Anti phospholipid marker
Diagnostic Test: Serum creatinine and Alb/create ratio
Other: 2019 European League Against Rheumatism/American College of Rheumatology classification criteria for systemic lupus erythematosus.
Other: SLEDAI scores
Registration Number
NCT06294483
Lead Sponsor
Sohag University
Brief Summary

The present study aims to:

Compare clinical features, hematological indices and disease activity between the early-onset and late-onset patients with systemic lupus erythematosus.

Evaluate the relationship between hematological indices (mean platelet volume, platelet lymphocyte ratio and neutrophil lymphocyte ratio) and Systemic lupus erythematosus (SLE) disease manifestations and activity.

Detailed Description

This is a cross sectional study, patients with SLE will be gathered from the Internal medicine department and Rheumatology and Immunology outpatient clinic in Sohag university hospital. All patients fulfilled 2019 European League Against Rheumatism/American College of Rheumatology classification criteria for systemic lupus erythematosus.

In this study, 100 SLE patients will be classified into two groups:

Group A: early onset SLE (age at diagnosis \< 50 years). Group B: late onset SLE. (age at diagnosis ≥ 50

Data collection procedure:

The following clinical data will be collected:

Clinical assessment:

Name, age, gender, smoker or ex-smoker or non-smoker, blood pressure and body mass index.

Clinical manifestations as:

Malar rash. Discoid rash. Photosensitivity. Mucocutaneous or oral ulcer. Alopecia. Raynaud's phenomena. History of deep venous thrombosis. Cutaneous vasculitis. Fever. Lupus nephritis. Arthritis. Myositis. Secondary antiphospholipid syndrome. Serositis. Pleural effusion. Renal manifestations (puffiness and lower limb edema). Neurological (headache, seizers, psychosis and Disturbed conscious level)

Hematological manifestations:

Thrombocytopenia (bleeding tendency) Anemia and Hemolytic anemia (anemic manifestation). Hypertension. Diabetes mellitus. Previous coronary event or Peripheral vascular disease.

Laboratory assessment:

1. CBC with differential WBCs count.

2. Antinuclear Antibody tests (ANA).

3. Anti-double-stranded DNA (ds DNA).

4. Anti-Sm.

5. C3 and C4 complement level.

6. Serum creatinine level.

7. Anti phospholipid marker (if needed). Each clinical data and laboratory results will be put into the SLEDAI score. The score is considered accurate and reliable. Categories of disease activity based on SLEDAI scores are as follows: no activity (SLEDAI= 0), mild activity (SLEDAI= 1-5), moderate activity (SLEDAI= 6-10), high activity (SLEDAI= 11-19) and very high activity (SLEDAI= 20).

The present study aims to:

Compare clinical features, and disease activity as SLEDAI score between the early-onset and late-onset patients with systemic lupus erythematosus.

Evaluate the relationship between hematological indices (mean platelet volume, platelet lymphocyte ratio and neutrophil lymphocyte ratio) and Systemic lupus erythematosus (SLE) disease manifestations and activity

Duration of study:

Six months after approval of the protocol by Medical Research Ethics Committee of Sohag faculty of medicine.

Inclusion criteria:

All patients fulfilled 2019 European League Against Rheumatism/American College of Rheumatology classification criteria for systemic lupus erythematosus.

Exclusion Criteria:

Patients received glucocorticoid or immunosuppressant medication. Patients presented with other chronic inflammatory diseases, infection, or other autoimmune diseases at the time of diagnosis Malignancy Pregnancy.

Recruitment & Eligibility

Status
ENROLLING_BY_INVITATION
Sex
All
Target Recruitment
100
Inclusion Criteria
  • All patients fulfilled 2019 European League Against Rheumatism/American College of Rheumatology classification criteria for systemic lupus erythematosus.
Exclusion Criteria
  • Patients received glucocorticoid or immunosuppressant medication. Patients presented with other chronic inflammatory diseases, infection, or other autoimmune diseases at the time of diagnosis Malignancy Pregnancy.

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
early onset SLESerum creatinine and Alb/create ratiogroup of patients diagnosed as SLE before age of fifty years old
early onset SLE2019 European League Against Rheumatism/American College of Rheumatology classification criteria for systemic lupus erythematosus.group of patients diagnosed as SLE before age of fifty years old
early onset SLESLEDAI scoresgroup of patients diagnosed as SLE before age of fifty years old
late onset SLECBCPatients were diagnosed as SLE at age of fifty years old or more
late onset SLESLEDAI scoresPatients were diagnosed as SLE at age of fifty years old or more
early onset SLEAntinuclear Antibody tests (ANA)group of patients diagnosed as SLE before age of fifty years old
early onset SLERest of ANA profilegroup of patients diagnosed as SLE before age of fifty years old
late onset SLERest of ANA profilePatients were diagnosed as SLE at age of fifty years old or more
early onset SLECBCgroup of patients diagnosed as SLE before age of fifty years old
early onset SLEC3 and C4 complement level.group of patients diagnosed as SLE before age of fifty years old
early onset SLEAnti phospholipid markergroup of patients diagnosed as SLE before age of fifty years old
late onset SLEAnti phospholipid markerPatients were diagnosed as SLE at age of fifty years old or more
late onset SLESerum creatinine and Alb/create ratioPatients were diagnosed as SLE at age of fifty years old or more
late onset SLE2019 European League Against Rheumatism/American College of Rheumatology classification criteria for systemic lupus erythematosus.Patients were diagnosed as SLE at age of fifty years old or more
late onset SLEAntinuclear Antibody tests (ANA)Patients were diagnosed as SLE at age of fifty years old or more
late onset SLEC3 and C4 complement level.Patients were diagnosed as SLE at age of fifty years old or more
Primary Outcome Measures
NameTimeMethod
Evaluate the correlation between hematological indices (mean platelet volume, platelet lymphocyte ratio and neutrophil lymphocyte ratio) and Systemic lupus erythematosus (SLE) disease manifestations and activity.for 2 weeks after admission of patient in sohag university hospital

After detection of previous outcome \[ disease activity , clinical feature,haematological indices \] we will compare them to detect the correlation between them for every patient

Evaluate the hematological indices as (mean platelet volume) of our SLE patient.for 2 weeks after admission of patient in sohag university hospital]

Neutrophils, lymphocytes, and platelets play important roles in the course of various diseases . In recent years, there has been a growing interest in the role of hematological indices (mean platelet volume, platelet lymphocyte ratio and neutrophil lymphocyte ratio) to estimate disease activity in some auto-immune diseases as and SLE . Neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), as CBC parameters, have recently shown to be useful markers of inflammation in autoimmune and inflammatory disorders so we will do CBC for our pt to detect mean platelet volume

Evaluate the hematological indices ( platelet lymphocyte ratio ) of our SLE patient.for 2 weeks after admission of patient in sohag university hospital]

Neutrophils, lymphocytes, and platelets play important roles in the course of various diseases . In recent years, there has been a growing interest in the role of hematological indices (mean platelet volume, platelet lymphocyte ratio and neutrophil lymphocyte ratio) to estimate disease activity in some auto-immune diseases as and SLE . Neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), as CBC parameters, have recently shown to be useful markers of inflammation in autoimmune and inflammatory disorders so we will do CBC for our pt to detect platelet lymphocyte ratio

Compare the degree of disease activity between the early-onset and late-onset patients with systemic lupus erythematosus.for 2 weeks after admission of patient in sohag university hospital]

Each clinical data and laboratory results will be put into the SLEDAI score. The score is considered accurate and reliable. Categories of disease activity based on SLEDAI scores are as follows: no activity (SLEDAI= 0), mild activity (SLEDAI= 1-5), moderate activity (SLEDAI= 6-10), high activity (SLEDAI= 11-19) and very high activity (SLEDAI= 20).

Evaluate the hematological indices as (neutrophil lymphocyte ratio) of our SLE patient.for 2 weeks after admission of patient in sohag university hospital]

Neutrophils, lymphocytes, and platelets play important roles in the course of various diseases . In recent years, there has been a growing interest in the role of hematological indices (mean platelet volume, platelet lymphocyte ratio and neutrophil lymphocyte ratio) to estimate disease activity in some auto-immune diseases as and SLE . Neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), as CBC parameters, have recently shown to be useful markers of inflammation in autoimmune and inflammatory disorders so we will do CBC for our pt to detect this neutrophil lymphocyte ratio

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Sohag university hospital

🇪🇬

Sohag, Egypt

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