Phenotypic and Genotypic Variations of Enterococcus Spp. Isolated From Patients With Hospital Acquired Infections in Sohag University Hospitals, Egypt
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Isolation and identification of Enterococci from different clinical samples
研究概览
简要总结
Enterococci are Gram-positive facultative anaerobic cocci arranged in short and medium chains. Enterococci reside in the gastrointestinal tract and usually function commensally with humans. They can, however, cause several infections, such as urinary tract infections (UTIs), intra-abdominal infection, bacteremia, or endocarditis.
Among many species identified, E. faecalis and E. faecium are the most common species capable of causing infection and posing a threat of antimicrobial resistance, with E. faecalis accounting for the majority of infections.
详细描述
Enterococci are Gram-positive facultative anaerobic cocci arranged in short and medium chains.
Enterococci reside in the gastrointestinal tract and usually function commensally with humans. They can, however, cause several infections, such as urinary tract infections (UTIs), intra-abdominal infection, bacteremia, or endocarditis.
Among many species identified, E. faecalis and E. faecium are the most common species capable of causing infection and posing a threat of antimicrobial resistance, with E. faecalis accounting for the majority of infections.
Pathogenic species of enterococci express many virulence factors such as adhesins, gelatinase, Enterococcus surface protein, aggregation substances and cytolysins along with biofilm formation. These factors enhance the ability of the pathogen to invade, attach and survive through the acquisition of nutrients in the host tissue. Their presence in drug resistant strains increases the severity of the infection
Enterococci are intrinsically resistant to antibiotics such as aminoglycosides and β-lactam-based antibiotics. Moderate resistance to aminoglycosides is due to the intrinsic low permeability of the enterococcal cell wall to the large aminoglycoside molecules and is more prevalent in E. faecium than E. faecalis. Intrinsic β-lactam resistance is due to the overexpression of penicillin-binding proteins with low affinity for β-lactams, which makes E. faecalis more resistant to penicillin than E. faecium
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 1 Month 至 90 Years（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •All patients suffering from infections that can be caused by Enterococci.
排除标准
- •All patients suffering from infections that aren't caused by Enterococci
结局指标
主要结局
Isolation and identification of Enterococci from different clinical samples
时间窗: from April 2023 to December 2023
Isolation and identification of Enterococci from different clinical samples (pus, urine, urinary catheter samples, stool, wound swabs, sputum, central venous catheter samples) will be collected under aseptic precautions from patients admitted at different departments at Sohag university hospitals.
Demonstration of Enterococci antibiotic susceptibility profile
时间窗: from April 2023 to December 2023
Strains confirmed as Enterococci will be examined for their antibiotic susceptibility by modified Kirby Bauer's disc diffusion method on Mueller-Hinton Agar.
Detection of strains of Enterococci which produce biofilm
时间窗: from April 2023 to December 2023
The biofilm formation activity of Enterococci isolates will be tested using the microtiter plate technique
Studying the effect of nanoparticles on Enterococci antimicrobial resistance pattern
时间窗: from April 2023 to December 2023
Inhibition zones of all groups will be compared to detect the effect of nanoparticles on Enterococci antimicrobial resistance pattern.
Studying the effect of nanoparticles on Enterococci biofilm producing ability
时间窗: from December 2023 to March 2024
The effect of nanoparticles on Enterococci biofilm producing ability
Genotypic characterization of Enterococci.
时间窗: rom December 2023 to March 2024
Molecular identification of some virulence factors genes and antibiotic resistance genes of Enterococci using polymerase chain reaction.
次要结局
未报告次要终点
研究者
Mai Gamal Abdel Naser Abbas Khalifa
principal investigator
Sohag University