Precision High-Intensity Training Through Epigenetics (PHITE)
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 239
- 试验地点
- 1
- 主要终点
- Change in muscle mass
研究概览
简要总结
The PHITE collaborative team, consisting of Tim Broderick, MD (Overall PI), Wright State University (WSU); Marcas Bamman, PhD (Site PI), The University of Alabama at Birmingham (UAB); and Ron Evans, PhD and Joe Ecker, PhD, Salk Institute for Biological Studies, are working with funding from the US Department of Defense (DoD), Office of Naval Research (ONR), to explore the link between physical training and epigenetics. This is a key interest area for the DoD ONR because it provides high-impact optimization of force readiness in warfighters with diverse backgrounds.
The term epigenetics refers to heritable changes in gene expression (active versus inactive genes) that does not involve changes to the underlying DNA sequence; a change in phenotype (the set of observable characteristics of an individual resulting from the interaction of its genotype with the environment) without a change in genotype (the genetic constitution of an individual organism) . This in turn affects how cells read the genes. Epigenetic change is a regular and natural occurrence but can also be influenced by several factors including age, the environment/lifestyle, and disease state. Epigenetic modifications can manifest as commonly as the manner in which cells terminally differentiate to end up as skin cells, liver cells, brain cells, etc. New and ongoing research is continuously uncovering the role of epigenetics in a variety of human conditions. This study is designed to assess whether epigenetics is a primary mechanism modulating how individuals adapt to specific exercise training prescriptions designed to produce a warfighter phenotype.
The PHITE team is organized around a shared test population of human subjects for which UAB will oversee recruitment, training, testing, and sampling. Healthy but untrained volunteers, both men and women, 18-27 y of age-phenotypical of the US warfighter-will participate in a 12-wk, two-arm, single-blind, randomized, exercise dose-response trial comparing two intensities of combined training: Moderate-Intensity vs. High-Intensity. Biospecimens are collected before and after an acute exercise bout in the pre-training state, and again after 12 weeks of 3 d/wk combined exercise training. Numerous phenotyping assessments are collected before after the 12-week intervention period to associate exercise training outcomes with molecular changes in the skeletal muscle and blood biospecimens.
详细描述
Overview:
The US military relies on physical training to increase and maintain force readiness. In this study, we hypothesize that epigenetic modification is a primary mechanism by which specific physical exercise training prescriptions transduce warfighter genotype into warfighting phenotype. Our aim with this program is 3- fold. We will (1) identify training methodologies that modify epigenetic responses; (2) characterize epigenetic regulation of physiological processes, pathways and mechanisms associated with moderate and high-intensity physical training, and (3) produce real-time biomarkers of cardiorespiratory and neuromuscular performance that predict physical training outcomes. We will satisfy these goals through the following six research projects:
I. Phenotyping the Exercise Dose-Response
- Determine the effect of exercise Intensity on neuromuscular and cardiorespiratory performance
- Assess the impact of baseline, pre-training phenotype on acute responsiveness and adaptability to different intensity exercise regimens
- Determine the effect of exercise intensity on mechanisms regulating muscle protein metabolism, myofiber hypertrophy, and muscle oxidative capacity
II. Real-time Genetic and Epigenetic Biomarkers of Performance
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Double (Investigator, Outcomes Assessor)
盲法说明
Outcomes assessors analyzing biospecimen samples are blinded to the arm to which each participant has been randomly assigned when doing analysis.
Investigators are blinded to each participant's randomized assignment.
入排标准
- 年龄范围
- 18 Years 至 27 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy 18-27 year olds who are not in the US armed forces
排除标准
- •History of regular endurance or resistance training in past 12 months
- •Inability to tolerate intense exercise
- •Uncontrolled hypertension
- •Unstable or exercise induced angina pectoris or myocardial ischemia
- •Vascular disease
- •Neurologic disease
- •Musculoskeletal disorder
- •Mental health disorder
- •Any chronic or infectious disease that would preclude full participation
- •Lidocaine allergy
- •Pregnancy
研究组 & 干预措施
Moderate Intensity (MOD)
Endurance Training (ET): 3 d/wk x 30 minutes (min) of steady-state, moderate-intensity exercise on a treadmill or stationary cycle ergometer at target heart rate (HR) = 65-75% of maximum oxygen consumption rate (VO2max). The two modes (treadmill, bicycle) are offered for variety, and each subject is required to use each mode at least 1 d/wk to prevent bias.
Resistance training (RT): 2 d/wk consisting of a prescription engaging all major muscle groups in 10 movements. Excluding abdominal crunches, target intensity is 12 repetitions/set to volitional fatigue. Subjects complete 3 sets of each movement, with ~60 seconds (s) rest between sets. For each movement, resistance increases when 14 repetitions are achieved for 2 of 3 sets.
HR is monitored throughout each session and stored for analysis.
干预措施: Moderate Intensity (Behavioral)
High Intensity (HI)
RT: The 2 d/wk RT prescription differs from the MOD arm only in intensity and rest intervals. The same approach to progression applies, but HI RT intensity targets 8-10 repetitions per set; thus, resistance loads increase when 10 repetitions are achieved for 2 of 3 sets. The HI arm performs "superset" training, pairing movements stressing different muscle groups, with only 30-45 s between.
ET: In lieu of steady-state endurance exercise, the HI arm performs high-intensity interval training (HIIT) 3 d/wk using a mix of challenging, explosive movements at maximal intensity. 10 x 30 s maximal intensity intervals are separated by 30 s rest intervals.
HR is monitored throughout each session and stored for analysis.
干预措施: High Intensity (Behavioral)
结局指标
主要结局
Change in muscle mass
时间窗: 16 weeks
Muscle mass is assessed using DXA (dual-energy X-ray absorptiometry)
Change in cardiorespiratory fitness (VO2max)
时间窗: 16 weeks
VO2max is assessed using via indirect calorimetry during a graded cycle ergometry protocol
次要结局
未报告次要终点
研究者
Marcas M. Bamman, PhD
Site Principle Investigator
University of Alabama at Birmingham