Research on Intestinal Flora and Metabonomics of Appendicitis in Children
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Fecal untargeted metabolomics testing
研究概览
简要总结
The goal of this observational study is to learn about the key bacterial flora and metabolites associated with appendicitis in children. The main questions it aims to answer are:
- To screen out the key biomarkers of pediatric appendicitis.
- What are the microbial differences in different parts of pediatric appendicitis patients.
Participants will detect feces using 16s ribosomal RiboNucleicAcid (16S rRNA) gene sequencing technology and differences of the fecal metabolites between healthy children and appendicitis children were analyzed using untargeted metabolomics based on Liquid chromatography-tandem mass spectrometry(LC-M S/MS) platform.Through the analysis of intestinal bacterial flora and metabolomics association and the differential analysis of intestinal bacterial flora in different parts of the case group, the key bacterial flora and metabolites were excavated.
详细描述
Participants will detect feces using 16s ribosomal RiboNucleicAcid (16S rRNA) gene sequencing technology and differences of the fecal metabolites between healthy children and appendicitis children were analyzed using untargeted metabolomics based on Liquid chromatography-tandem mass spectrometry(LC-M S/MS) platform.
Differences in the intestinal flora of different parts of the case group: the intestinal flora of the case group in feces and rectum were detected by 16s ribosomal RiboNucleicAcid (16S rRNA) gene sequencing technology, and the intestinal flora of different parts were analyzed and compared.
Through the analysis of intestinal bacterial flora and metabolomics association and the differential analysis of intestinal bacterial flora in different parts of the case group, the key bacterial flora and metabolites were excavated.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 1 Year 至 14 Years（Child）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Children aged 1-14 years who were initially diagnosed with appendicitis and underwent laparoscopic appendectomy
- •Children aged 1-14 years with non-digestive tract disease and no history of digestive tract disease
排除标准
- •A family history of intestinal disease
- •Allergic to antibiotic therapy drugs
- •Patients with long-term drug use causing microbial structure changes or intestinal flora disorders
- •Patients with other congenital diseases such as Meckel's diverticulum, intestinal rotation and other malrotation
- •With other systemic diseases, such as obvious upper respiratory tract infection or myocarditis
- •Children with other digestive system diseases after auxiliary examination or surgical diagnosis
结局指标
主要结局
Fecal untargeted metabolomics testing
时间窗: up to 24 weeks
To analyze the fecal metabolite differences and the correlation of appendicitis and metabolites using untargeted metabolomics based on the LC-M S/MS platform. To screen key metabolites in pediatric appendicitis.
The 16S rRNA gene sequence
时间窗: up to 24 weeks
Samples were qualified for Polymerase Chain Reaction(PCR) amplification of the V3 and V4 variable regions of the 16 rRNA gene. The Polymerase Chain Reaction(PCR) products were then quantified by confirming the presence of PCR amplicons by gel electrophoresis on a 2% agarose gel and staining with ethidium bromide, and equimolar amounts (100 ng) of PCR amplicons were pooled prior to pyrosequencing. High-throughput sequencing of the qualified 16S amplicon libraries.
次要结局
未报告次要终点
研究者
HongyiQu
professor
Qianfoshan Hospital