Investigation of Microbiome-Derived Metabolite Production in Response to a Mediterranean Diet
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Laval University
- Enrollment
- 21
- Locations
- 2
- Primary Endpoint
- Microbiome-derived metabolite profiling
Study Overview
Brief Summary
This project proposes to characterise the microbiome-derived metabolites produced upon ingestion of bioactive precursor-rich meals by healthy volunteers in order to investigate the relationship between gut microbiome composition and the nature of dietary metabolites production.
Detailed Description
There is a large inter-individual variability in gut microbiome-derived metabolites production upon ingestion of similar meals. Since many of these microbiome-derived metabolites have enteric and systemic biological effects, these variabilities could in part account for the different responses to meals and diets.
This project thus aims to improve the understanding the relationship between gut microbiome composition and the nature of dietary metabolites produced from similar bioactive precursor-rich meals.
The investigators therefore propose to characterise the circulating microbiome-derived metabolite profiles in healthy individuals fed the same 2-day precursor-rich Mediterranean diet prior to and after a 2-week of habitual Canadian diet feeding.
Study Design
- Study Type
- Interventional
- Allocation
- Na
- Intervention Model
- Single Group
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 35 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Men and women aged between 18 and 35 years
- •Body mass index between 18.5 and 30 kg/m2
- •General good health
- •Computer and Internet access
- •Basic understanding of written French
Exclusion Criteria
- •Intestinal pathologies including inflammatory bowel disease and gastrointestinal cancers
- •Pregnant and/or breast-feeding women
- •Alcohol consumption greater than 15 portions for men and 10 for women weekly
- •Active tobacco usage
- •Consumption of dietary supplements (e.g. multivitamins, omega-3, probiotics)
- •Completion of a course of antibiotics in the past 3 months
- •Regular use of anti-inflammatory drugs, or use in the past 3 months
- •Important weight change (+/- 5 kg) in the past 6 months
- •Allergies or food intolerances to ingredients present in trial diets
Outcomes
Primary Outcomes
Microbiome-derived metabolite profiling
Time Frame: 19 days
Plasma and urine metabolomics profiling of gut-microbiome derived metabolites (e.g., branched-chain amino acids \[BCAA\], short chain fatty acids \[SCFA\], trimethylamine \[TMA\], amino acids- and polyphenol-metabolites) will also performed at the same time points.
Gut microbiome characterisation
Time Frame: 19 days
Fecal microbiome composition will be characterise by rRNA 16S sequencing prior to and following both 2-day Mediterranean diet periods. Relevant indexes of microbiome diversity (e.g. Shannon beta-diversity), ratios between meaningful bacterial markers of gut dysbiosis (e.g. Firmicutes:Bacteroidetes) and specific microbiome differences in bacterial Families, Genera or species between study periods and individuals.
Secondary Outcomes
- Endocannabinoid interaction with interindividual microbiome composition(19 days)
Investigators
Alain Veilleux
Professor
Laval University