Effect of Platelet Storage on Complement Activation
- Conditions
- Platelet Storage
- Registration Number
- NCT03280823
- Lead Sponsor
- Shimaa Abdeleem
- Brief Summary
Platelet units are stored and complement factors are evaluated
- Detailed Description
Ten units of platelets will be selected from the regular inventory of the Central Blood Transfusion Services/Assiut University Hospitals . All units of platelets will be collected, processed, and tested following the standard procedures.
Thesis units will be stored on a platelet rotator with the temperature controlled between 22 and 24c. Samples will be obtained using a sterile technique .
The investigators will take basal sample and samples on day 2,3,4 and 5. Complement components will be assayed : C4 (classical pathway), C3 (common pathway) and C5 (terminal pathway).
This evaluation will be done using ELISA technique
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 10
Inclusion Criteria
- Negative screening and confirmatory tests will be done for all platelet donors
Exclusion Criteria
- Use of aspirin or non steroidal anti-inflammatory drugs or medication affecting the platelets.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Platelets can be used after storage upto 5 days instead of using in the same day of donation without complement activation. 5 days
- Secondary Outcome Measures
Name Time Method