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Post-transfusion Platelet Count

Completed
Conditions
Leukemia
Multiple Sclerosis
Interventions
Biological: Platelet transfusion
Registration Number
NCT02601131
Lead Sponsor
Region Skane
Brief Summary

The purpose of this study is to identify how the platelet count, complement system and endothelial markers are affected over time, after platelet transfusion in 4 different hematological patient groups.

Detailed Description

Patients who are hospitalized at the Clinic for Hematology Diseases at the Skåne University Hospital (SUS) in Lund, Sweden with a central venous catheter or port-a-cath and should receive platelet transfusion due to thrombocytopenia are asked to participate in the study. Some patients who are being considered for platelet transfusions on several occasions will be able to attend more than one time in this study. After signed informed consent blood samples are drawn from the central line before, immediately after and then approximately. 1, 4, 8, 16 and 24 hours following the platelet transfusion. The platelet count (PLC) will then be taken daily following 4 days.

Sample size. Data from previous studies (Norol-98) shows that the PLC after platelet transfusion rises by 33 ± 25 x109/L (mean ± SD) 12 hours post-transfusion in patients with acute myeloid leukemia or pre-conditioning prior to bone marrow transplantation. The investigators want to show the same rise in the PLC after 12 hours with 80% power and 5% alpha risk of error for each group resulting in the sample size 13 per group and a total of 65 patients.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
80
Inclusion Criteria
  • Patients admitted to the Clinic of Hematology Diseases, Skåne University Hospital, Lund, Sweden belonging to either of the groups described in paragraph 8 above and are prescribed platelet transfusion.
Exclusion Criteria
  • Patients with aplastic anemia or treatment with antithymocyte globulin (ATG)

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Autologous stem cell transplantationPlatelet transfusionPatients undergoing autologous stem cell transplantation
Platelet transfusion prophylaxisPlatelet transfusionPatients receiving platelet transfusion prophylaxis before the intervention, such as insertion of a central venous catheter or lumbar puncture
Allogeneic stem cell transplantationPlatelet transfusionPatients undergoing allogeneic stem cell transplantation including both myeloablative conditioning (MAC) and the reduced-intensity conditioning (RIC)
AML, ALL and MDSPlatelet transfusionPatients receiving intensive chemotherapy with diagnoses of acute myeloid leukemia (AML), acute lymphocytic leukemia (ALL) or myelodysplastic syndromes (MDS).
Primary Outcome Measures
NameTimeMethod
Change in platelet count after platelet transfusion4 days after platelet transfusion
Secondary Outcome Measures
NameTimeMethod
Change in concentrations of complement factors in plasma after platelet transfusion4 days after platelet transfusion
Change in concentrations of complement factors in platelet rich plasma after platelet transfusion4 days after platelet transfusion
Change in concentrations of endothelial markers in plasma after platelet transfusion4 days after platelet transfusion
Analysis of platelets in platelet transfusions given to patients included in the study4 days after platelet transfusion

Flow cytometry of platelets in platelet transfusions given to patients included in the study

Trial Locations

Locations (1)

Region Skåne

🇸🇪

Lund, Sweden

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