Prognostic Multigene Expression Classification of Neuroblastoma Patients
概览
- 阶段
- 不适用
- 干预措施
- 未指定
- 疾病 / 适应症
- Localized Resectable Neuroblastoma
- 发起方
- Children's Oncology Group
- 入组人数
- 350
- 试验地点
- 1
- 主要终点
- mRNA expression levels from genes that have been shown (in at least two independent studies) to have power for outcome prediction in children with NB, assessed by real-time PCR
- 状态
- 已完成
- 最后更新
- 9年前
概览
简要总结
This research trial studies specimens from young patients with neuroblastoma. Studying the genes expressed in specimens from patients with cancer may help doctors identify biomarkers related to cancer. It may also help doctors predict how patients will respond to treatment.
详细描述
PRIMARY OBJECTIVES: I. Establish a robust messenger ribonucleic acid (mRNA) gene expression classifier for improved outcome prediction in children with neuroblastoma by using real-time polymerase chain reaction (PCR) to quantify mRNA levels from genes that have been shown in at least 2 independent studies to have predictive power. II. Identify microRNA (miRNA) patterns that have prognostic significance in neuroblastoma (NB) as accumulating evidence indicates that alterations in miRNA expression play a critical role in tumorigenesis and can be used in prognostic evaluation. III. To perform an integrated analysis of the established microRNA classifier with our mRNA signature to determine whether it results in even better classification of NB tumors. OUTLINE: Previously collected samples are analyzed for 59 prognostic genes by real-time quantitative PCR-based gene expression profiling.
研究者
入排标准
入选标准
- •Diagnosis of primary Neuroblastoma
- •Untreated disease
- •RNA samples from primary untreated NB-tumors available in Children's Oncology Group (COG) repository
- •At least 60% tumor cells
- •At least 2 years of follow-up from the date of diagnosis
- •Full clinical and biological annotation
排除标准
- 未提供
结局指标
主要结局
mRNA expression levels from genes that have been shown (in at least two independent studies) to have power for outcome prediction in children with NB, assessed by real-time PCR
时间窗: Baseline
Multivariate logistic regression analyses will be performed to determine if the mRNA expression signature is a significant independent predictor after controlling for currently used risk factors.
miRNA patterns that have prognostic significance in NB, assessed by a reverse transcription quantitative PCR-based 'stem-loop' method to quantify expression levels
时间窗: Baseline
Multivariate logistic regression analyses will be performed to determine if the miRNA expression signature is a significant independent predictor after controlling for currently used risk factors.