Analysis of the Pre- and Post-prandial Levels of Appetite Regulatory Hormones in Adult Individuals
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Enrollment
- 66
- Locations
- 1
- Primary Endpoint
- plasma leptin concentration
Study Overview
Brief Summary
The present study aims to evaluate in adults with normal weight and with overweight or obesity who live in the city of La Plata the circulating levels of various hormones that regulate appetite and the gene expression of their receptors in peripheral blood mononuclear cells before and after food intake.
Detailed Description
the investigators plan a single test meal study in males and females with normal weight (NW) or overweight/obesity (OW/OB) assessing whether plasma appetite regulatory hormones levels are associated with different metabolic and anthropometric variables as well as with appetite sensations.
Study Design
- Study Type
- Interventional
- Allocation
- Na
- Intervention Model
- Single Group
- Primary Purpose
- Prevention
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 50 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •adult individuals body mass index> 18.5 kg∙m-2
Exclusion Criteria
- •chronic medical illness, personal history of diabetes, history of medical condition, or medication related to obesity or diabetes risk status.
Arms & Interventions
food intake (breakfast)
the breakfast composition is 56 kcal% carbohydrates, 12 kcal% protein and 34 kcal% lipids and comprises yogurt, cereals and almonds
Intervention: food (breakfast) (Dietary Supplement)
Outcomes
Primary Outcomes
plasma leptin concentration
Time Frame: 1 hour post -prandial
leptin concentration (ug/ml)
plasma glucagon-like peptide 1 concentration
Time Frame: 1 hour post -prandial
glucagon-like peptide 1 concentration (ug/ml)
plasma ghrelin concentration
Time Frame: 1 hour post -prandial
ghrelin concentration (fmol/ml)
plasma des-acyl ghrelin concentration
Time Frame: 1 hour post -prandial
des-acyl ghrelin concentration (fmol/ml)
ghrelin deacylation
Time Frame: 1 hour post -prandial
plasma desacylase actvitity of ghrelin (relative units)
hunger sensation
Time Frame: pre-prandial and 15, 30, 45 and 60 min post-prandial
visual analog scale (cm)
plasma liver antimicrobial peptide 2 concentration
Time Frame: 1 hour post -prandial
liver antimicrobial peptide 2 concentration (pmol/ml)
plasma glucose concentration
Time Frame: 1 hour post -prandial
glucose concentration (mg%)
insulin receptor gene expression in peripheral blood mononuclear cells
Time Frame: pre-prandial
insulin receptor gene expression in peripheral blood mononuclear cells (relative expression)
"prospective food consumption" sensation
Time Frame: pre-prandial and 15, 30, 45 and 60 min post-prandial
visual analog scale (cm)
plasma triglycerides concentration
Time Frame: 1 hour post -prandial
triglycerides concentration (mg%)
leptin receptor gene expression in peripheral blood mononuclear cells
Time Frame: pre-prandial
leptin receptor gene expression in peripheral blood mononuclear cells (relative expression)
ghrelin receptor gene expression in peripheral blood mononuclear cells
Time Frame: pre-prandial
ghrelin receptor gene expression in peripheral blood mononuclear cells (relative expression)
plasma total cholesterol concentration
Time Frame: 1 hour post -prandial
total cholesterol concentration (mg%)
ghrelin proteolysis
Time Frame: 1 hour post -prandial
plasma proteolytic activity of ghrelin (relative units)
fullness sensation
Time Frame: pre-prandial and 15, 30, 45 and 60 min post-prandial
visual analog scale (cm)
"desire to eat" sensation
Time Frame: pre-prandial and 15, 30, 45 and 60 min post-prandial
visual analog scale (cm)
plasma ghrelin concentration
Time Frame: pre -prandial
ghrelin concentration (fmol/ml)
plasma leptin concentration
Time Frame: pre -prandial
leptin concentration (ug/ml)
plasma glucagon-like peptide 1 concentration
Time Frame: pre -prandial
glucagon-like peptide 1 concentration (ug/ml)
plasma liver antimicrobial peptide 2 concentration
Time Frame: pre -prandial
liver antimicrobial peptide 2 concentration (pmol/ml)
plasma des-acyl ghrelin concentration
Time Frame: pre -prandial
des-acyl ghrelin concentration (fmol/ml)
plasma glucose concentration
Time Frame: pre -prandial
glucose concentration (mg%)
plasma triglycerides concentration
Time Frame: pre-prandial
triglycerides concentration (mg%)
plasma total cholesterol concentration
Time Frame: pre -prandial
total cholesterol concentration (mg%)
ghrelin proteolysis
Time Frame: pre -prandial
plasma proteolytic activity of ghrelin (relative units)
ghrelin deacylation
Time Frame: pre -prandial
plasma desacylase actvitity of ghrelin (relative units)
Secondary Outcomes
No secondary outcomes reported