Acute Whey Protein And Casein Supplementation: Effect On Protein Metabolism After Resistance Exercise

Not Applicable

Completed

• Conditions: Protein Supplementation; Sports Nutrition
• Interventions: Dietary Supplement: Blend Protein Supplementation; Dietary Supplement: Placebo Supplementation; Other: Resistance Exercise

• Registration Number: NCT04648384
• Lead Sponsor: University of Sao Paulo

Brief Summary

The proteins of human breast milk are the most important endocrine signaling system that promotes neonatal growth by increasing the release of insulin, insulin-like growth factor-1 (IGF-1), and the leucine-mediated mammalian target of rapamycin complex 1- (mTORC1) signaling of pancreatic β-cells. Remarkably, the branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are involved in the growth-promoting effects of milk, protein biosynthesis, and metabolism because they physiologically stimulate insulin secretion The present study sought to investigate the effects of the combination of whey protein and casein in the ratio of 80:20 ("whey protein: casein" or "casein: whey protein") as breast milk proportion on the peak and the period of the permanence of branched-chain amino acids in the blood circulation, final metabolites of protein metabolism and delayed onset muscle soreness (DOMS) after a single bout of fasting or a resistance exercise session.

Detailed Description

The present study investigated the effects of the association of WP and casein in the ratio of 80:20, a similar ratio of human breast milk, on blood branched-chain amino acids (BCAAs) profile by HPLC technique, markers of protein metabolism (creatinine, urea, and urine nitrogen) and delayed onset muscle soreness (DOMS) after a single bout of resistance exercise. A double-blind, crossover and acute study was conducted with ten men (age 29 ± 8 y; BMI: 25.4 ± 2.9 Kg/m2; 77 ± 12 kg; 1.74 ± 0.09 m) that were randomly assigned to five supplementation treatments: WP - whey protein; CAS - casein; WP/CAS - 80% WP/20% CAS; CAS/WP - 80% CAS/ 20% WP; PLA - placebo. Participants were submitted to the following evaluations: performance sessions, blood collection for each session for the BCAAs profile determination, two food records, 3-days assessment of DOMS (24h, 48h, 72h) after each treatment.

Study Timeline

• Study Start: 2016-03-20
• Primary Completion: 2019-07-20
• Study Completion: 2019-10-20
• Status Verified: 2020-11
• Study First Submitted: 2020-11-09
• Study First Submitted QC: 2020-11-30
• Last Update Submitted: 2020-11-30
• Study First Posted: 2020-12-01
• Last Update Posted: 2020-12-02

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 10

Inclusion Criteria

• Healthy
• practice resistance training at least 3x a week

Exclusion Criteria

• Smokers
• Muscle injures
• cardiovascular diseases
• Diabetes mellitus
• Lactose intolerance

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Placebo Supplementation	Resistance Exercise	Placebo supplementation composed of 20 gram of Maltodextrin and fasting state 10 sets of 10 reps at 85% of 1 Maximum repetition
Protein Supplementation and Resistance exercise	Blend Protein Supplementation	Protein supplementation composed of 20 gram of whey protein or casein in different proportions (80:20) and fasting state 10 sets of 10 reps at 85% of 1 Maximum repetition
Protein Supplementation and Resistance exercise	Resistance Exercise	Protein supplementation composed of 20 gram of whey protein or casein in different proportions (80:20) and fasting state 10 sets of 10 reps at 85% of 1 Maximum repetition
Resistance Training	Resistance Exercise	Resistance Exercise will be performed before supplementation and is composed by 10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise.
Placebo Supplementation	Placebo Supplementation	Placebo supplementation composed of 20 gram of Maltodextrin and fasting state 10 sets of 10 reps at 85% of 1 Maximum repetition

Primary Outcome Measures

Name	Time	Method
Change from baseline in plasma amino acids concentration	5 weeks	Blood sample collected for analysis of BCAA concentration after blend protein or placebo supplementation expressed in (µmol/L)
Change from baseline in 1RM test	5 weeks	Change of training load using RPE, time and load expressed in (a.u)
Changes from baseline in macronutrient intake	5 weeks	Change of macronutrient intake were calculated pre and post treatment expressed in (g/24 hours)
Change in delayed onset muscle soreness	5 weeks	Change from baseline the delayed onset muscle soreness pre, post, 24 hours, 48 hours and 72 hours after treatment expressed in (a.u)
Change in protein metabolism	5 weeks	Change in nitrogen excretion, and nitrogen balance after protein supplementation expressed in (g/24 hours)

Trial Locations

Locations (1)

University of São Paulo, School of Physical Education and Sports of Ribeirão Preto.; 🇧🇷 Ribeirão Preto, São Paulo, Brazil