TwinLife: TWIN Longitudinal Investigation of FEtal discordance
Recruiting
- Conditions
- Monochorionic twin pregnancies
- Registration Number
- NL-OMON22667
- Lead Sponsor
- The Dutch Heart Foundation
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 200
Inclusion Criteria
MC twin pregnancies
- Parents aged = 18 years, who are able to consent
- Written informed consent from both parents to participate in this longitudinal study
Exclusion Criteria
- The presence of major anatomical abnormalities
- Triplet pregnancies or higher order multiple pregnancies
- Twin reversed arterial perfusion (TRAP)
- MC twins with single or double fetal demise
Study & Design
- Study Type
- Observational non invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method 1. Percentage difference in DNA methylation in MSCs at birth within MC twin pairs in relation to measures of intra-uterine discordance: percentage difference in birthweight and percentage difference in placenta share. <br>2. Within-twin pair differences at follow-up in childhood (2, 5, 8 years) with respect to risk of CVD and NDI as explained by the DNA methylation differences at birth. Study parameters of CVD at follow-up: cardiac load reflected by left-ventricular mass, vascular stiffness reflected by aortic pulse-wave velocity
- Secondary Outcome Measures
Name Time Method 1. Explore the persistence of epigenetic differences in peripheral tissues (buccal swabs) from birth to 2, 5 and 8 years follow-up.<br>2. Test the association of epigenetic changes in MSC cells with cellular metabolism.<br>3. Correlate within-pair differences in fetal cardiac growth and function and outcome at birth and at follow-up.