Observational study to investigate the regional differences in abdominal adipose tissue depots regarding function and morphology, in relation to systemic inflammation, endothelial dysfunction and atherosclerosis in patients undergoing elective abdominal aortic surgery.;korte naam: Adipose tissue Dysfunction in Patients in relation to ObeSity and vascular Events - study
Completed
- Conditions
- abdominal aortic aneurysm or localized widening (dilation) of a blood vessel1005254710002363
- Registration Number
- NL-OMON38295
- Lead Sponsor
- niversitair Medisch Centrum Utrecht
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 30
Inclusion Criteria
1.Written and signed informed consent.
2.Male or female (postmenopausal) patients
3.Indicated for elective abdominal aortic surgery in the University Medical Center Utrecht
Exclusion Criteria
1.Thyroid disease (TSH > 5 mU/L with clinical symptoms of hypothyroidism)
2.Hepatic disease (ASAT or ALAT > 2 times the upper limit of normal)
3.History of malignancy (in the last 2 years)
4.Use of thiazolidinediones (TZD)
5.Use of immune suppressive medication (equivalent of prednisolon >= 10 mg /day)
6.History of liposuction
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Differences in function and morphology between the distinct abdominal adipose<br /><br>tissue depots, by comparing:<br /><br>- The secretion profile of adipocytokines ex vivo measured with LUMINEX and<br /><br>ELISA<br /><br>- The mean difference in basal mRNA expression of adipocytokines of the adipose<br /><br>tissues, measured by quantitative RT-PCR and<br /><br>- The mean differences in morphology, measured by number and size of<br /><br>adipocytes, capillaries, inflammatory cells and ischemia.<br /><br>- The mean differences in number of specific inflammatory cells present in<br /><br>subcutaneous versus visceral adipose tissue.</p><br>
- Secondary Outcome Measures
Name Time Method <p>1. Mean differences in quantity of the distinct adipose tissue depots measured<br /><br>from images of routinely performed CT scans, correlated to differences in<br /><br>functional and/or morphological parameters of the adipose tissue depots like<br /><br>adipocytokine secretion pattern or mean adipocyte cell size.<br /><br><br /><br>2. Difference between independent correlations of the different AT depots with<br /><br>clinical parameters of the metabolic syndrome. Therefore functional and/or<br /><br>morphological parameters of each adipose tissue depot (like adipocytokine<br /><br>secretion pattern or mean adipocyte cell size) will be correlated to clinical<br /><br>parameters of the patient and correlations between the distinct adipose tissue<br /><br>depots will be compared.<br /><br><br /><br>3. Mean differences in function and morphology of the distinct adipose tissue<br /><br>depots, correlated to systemic plasma concentrations of bioactive mediators. </p><br>