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Major depression as a metabolic disorder: The role of oxygen homeostasis and mitochondrial bioenergetics in depression etiology and therapy

Not Applicable
Recruiting
Conditions
F32
F33
Depressive episode
Recurrent depressive disorder
Registration Number
DRKS00025457
Lead Sponsor
Department of Clinical and Biological Psychology, Ulm University
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Recruiting
Sex
All
Target Recruitment
300
Inclusion Criteria

A diagnosis of major depressive disorder according to DSM-5; accepted psychological comorbidities: anxiety disorders, and somatoform disorders. Stable medication is permitted.

Exclusion Criteria

High suicidality, PTSD and complex PTSD, Borderline personality disorder, eating disorders, obsessive compulsive disorder, bipolar disorder, schizophrenia or a history of psychotic disorders, acute substance-related disorder, organic mental disorder, pregnancy and nursing, neurological diseases, immunological and endocrine disorders, autoimmune diseases such as chronic viral infections, rheumatoid arthritis, cardiovascular comorbidities, cancer, chronic obstructive pulmonary diseases, Body Mass Index (BMI) > 30, usage of certain intrauterine devices (eg. Mirena)

Study & Design

Study Type
interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Primary outcomes will be measured using the whole blood and immune cells of the participants at the beginning of each time point. ATP-turnover-related respiration, uncoupled respiration, and routine respiration will be measured from the immune cells, using high resolution respirometry. Mitochondrial density of the immune cells will be determined spectrophotometrically by measuring citrate syntase activity of the immune cells. Hemoglobin concentration and erythrocyte cell number per ml will be measured in whole blood.
Secondary Outcome Measures
NameTimeMethod
Secondary outcomes will be measured using the whole blood and immune cells of the participants at the beginning of each time point. <br>Oxygen-homeostasis-related parameters will be determined either from whole blood with a blood gas analyzer (glucose, lactate, pH, O2 and CO2 partial pressure), or from serum by clinical routine measurements (free iron, iron proteins, bilirubin), or with pulse oximeter (O2 saturation) and ear thermometer (body temperature). <br>Inflammation parameters (CRP, IL-1beta, IL-2, IL-6, IL-10, TNF-alpha) will be determined from serum via enzyme-linked immunosorbent assay.<br>Oxidative stress parameters will be determined from whole blood via Electron Spin Resonance (Reactive Oxygen Species) and indirectly from serum via enzyme-linked immunosorbent assay (8OH-dG, 8-isoprostane).
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