Neuropeptide Y Regulates Neurogenic Pulp Inflammation

Not Applicable

Completed

• Conditions: Neurogenic Inflammation; Dental Pulp Disease
• Interventions: Other: H2O2 at 35% for 8 minutes; Other: H2O2 at 40% for 20 minutes; Other: H2O2 at 25% plus cold blue light for 15 minutes

• Registration Number: NCT06606236
• Lead Sponsor: Institucion Universitaria Colegios de Colombia

Brief Summary

Objective. This study assessed the neuropeptide Y (NPY) expression in healthy human dental pulp following tooth bleaching with three in-office hydrogen peroxide-based systems: Opalescence Boost© (Ultradent Products, South Jordan, UT), Pola Office© (SDI, Victoria, Australia), and Zoom© (Zoom! Bleaching System; Discuss Dental, Culver City, CA).

Materials and Methods. Forty dental pulps were collected from healthy premolars scheduled for extraction for orthodontic reasons. Teeth were divided into four groups containing ten healthy premolars each: Control group (n= 10): the teeth were not exposed to dental bleaching agents (healthy pulps assessed for normal/basal NPY values). Pola Office system group (n= 10): application of Pola office (35% H2O2) for 8 minutes. Opalescent Boost system group (n= 10): application of Opalescent Boost (40% H2O2) for 20 minutes. Zoom system group (n= 10): application of Zoom! (25% H2O2 + cold blue light) for 15 minutes. We rigorously followed the manufacturer\'s instructions for all bleaching systems. Following the extractions, the pulpal tissue was collected, placed in a 4% formaldehyde solution in Eppendorf tubes, and processed. NPY levels were measured using enzyme-linked immunosorbent assay (ELISA).

Detailed Description

Not available

Study Timeline

• Study Start: 2024-02-05
• Primary Completion: 2024-02-28
• Study Completion: 2024-02-28
• Status Verified: 2024-09
• Study First Submitted: 2024-09-14
• Study First Submitted QC: 2024-09-18
• Last Update Submitted: 2024-09-18
• Study First Posted: 2024-09-20
• Last Update Posted: 2024-09-20

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 40

Inclusion Criteria

• Dental pulps were collected from non-smoking donors
• Patients who need premolar extractions for orthodontic purposes.
• These premolars must meet the following requirements:
• Caries and restoration-free,
• Complete root development (visually and radiographically confirmed),
• Normal response to sensitivity testing.

Exclusion Criteria

• Evidence of periodontal disease,
• Traumatic occlusion
• Previous orthodontic force application.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Pola Office system group	H2O2 at 35% for 8 minutes	Application of Pola office n=10
Opalescent Boost system group	H2O2 at 40% for 20 minutes	Application of Opalescent Boost n=10
Zoom system group	H2O2 at 25% plus cold blue light for 15 minutes	Application of Zoom! n=10

Primary Outcome Measures

Name	Time	Method
Neuropeptide Y expression	8-20 minutes	Measurement of the increase or not of NPY expression compared to the control group (teeth without intervention)

Trial Locations

Locations (1)

Facultad de Odontologia, Unicoc; 🇨🇴 Bogota, Colombia