MedPath

Neuropeptide Y Regulates Neurogenic Pulp Inflammation

Not Applicable
Completed
Conditions
Neurogenic Inflammation
Dental Pulp Disease
Interventions
Other: H2O2 at 35% for 8 minutes
Other: H2O2 at 40% for 20 minutes
Other: H2O2 at 25% plus cold blue light for 15 minutes
Registration Number
NCT06606236
Lead Sponsor
Institucion Universitaria Colegios de Colombia
Brief Summary

Objective. This study assessed the neuropeptide Y (NPY) expression in healthy human dental pulp following tooth bleaching with three in-office hydrogen peroxide-based systems: Opalescence Boost© (Ultradent Products, South Jordan, UT), Pola Office© (SDI, Victoria, Australia), and Zoom© (Zoom! Bleaching System; Discuss Dental, Culver City, CA).

Materials and Methods. Forty dental pulps were collected from healthy premolars scheduled for extraction for orthodontic reasons. Teeth were divided into four groups containing ten healthy premolars each: Control group (n= 10): the teeth were not exposed to dental bleaching agents (healthy pulps assessed for normal/basal NPY values). Pola Office system group (n= 10): application of Pola office (35% H2O2) for 8 minutes. Opalescent Boost system group (n= 10): application of Opalescent Boost (40% H2O2) for 20 minutes. Zoom system group (n= 10): application of Zoom! (25% H2O2 + cold blue light) for 15 minutes. We rigorously followed the manufacturer\'s instructions for all bleaching systems. Following the extractions, the pulpal tissue was collected, placed in a 4% formaldehyde solution in Eppendorf tubes, and processed. NPY levels were measured using enzyme-linked immunosorbent assay (ELISA).

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
40
Inclusion Criteria
  • Dental pulps were collected from non-smoking donors
  • Patients who need premolar extractions for orthodontic purposes.
  • These premolars must meet the following requirements:
  • Caries and restoration-free,
  • Complete root development (visually and radiographically confirmed),
  • Normal response to sensitivity testing.
Exclusion Criteria
  • Evidence of periodontal disease,
  • Traumatic occlusion
  • Previous orthodontic force application.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Pola Office system groupH2O2 at 35% for 8 minutesApplication of Pola office n=10
Opalescent Boost system groupH2O2 at 40% for 20 minutesApplication of Opalescent Boost n=10
Zoom system groupH2O2 at 25% plus cold blue light for 15 minutesApplication of Zoom! n=10
Primary Outcome Measures
NameTimeMethod
Neuropeptide Y expression8-20 minutes

Measurement of the increase or not of NPY expression compared to the control group (teeth without intervention)

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Facultad de Odontologia, Unicoc

🇨🇴

Bogota, Colombia

© Copyright 2025. All Rights Reserved by MedPath