Optimization of Oocyte Warming Protocols
- Conditions
- Oocyte Cryopreservation
- Registration Number
- NCT07105891
- Lead Sponsor
- IRCCS San Raffaele
- Brief Summary
This is a retrospective study with a sibling design comparing new oocyte thawing protocols (rapid and ultra-rapid) with the traditional method
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- ACTIVE_NOT_RECRUITING
- Sex
- Female
- Target Recruitment
- 399
- Vitrified oocytes from the Centro Scienze della Natalità, IRCCS San Raffaele Hospital
- Patients who explicitly consented to the disposal of their stored gametes and authorized their use for research purposes through the informed consent process
- Oocytes cryopreserved using the vitrification technique
- Patients age 20-39 yo
- All oocytes cryopreserved using the slow freezing technique
- Oocytes from patients older than 40 years
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Primary Outcome Measures
Name Time Method Oocytes Survival rate 2 hours post-warming Oocyte survival rate will be assessed 2 hours after thawing using morphological evaluation under a light microscope. Surviving oocytes will be identified by the presence of an intact zona pellucida and oolemma, a round shape, and homogeneous cytoplasm without signs of lysis, fragmentation, or cytoplasmic granulation.
- Secondary Outcome Measures
Name Time Method Chromosome distribution 2 hours post thawing Chromosome distribution will be assessed using confocal microscopy following immunofluorescence staining. The distribution will be classified as normal, misaligned, or displaced.
Spindle configuration 2 hours post thawing Spindle configuration in thawed oocytes will be assessed using confocal microscopy following immunofluorescence staining. The spindles will be classified as normal, slightly aberrant, or aberrant.
Trial Locations
- Locations (1)
IRCCS San Raffaele Hospital
🇮🇹Milan, Lombardia, Italy
IRCCS San Raffaele Hospital🇮🇹Milan, Lombardia, Italy