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Study of UC-781 Vaginal Microbicide

Phase 1
Completed
Conditions
HIV Infections
Interventions
Drug: topical vaginal application of UC-781 gel
Registration Number
NCT00132444
Lead Sponsor
CONRAD
Brief Summary

This study will help determine whether the agent UC-781, formulated as a gel, is safe when applied to the vagina twice daily for 14 days. It will also assess whether women find the gel acceptable to use.

Detailed Description

Purpose: To assess the safety and acceptability of vaginal use of 0.1% and 0.25% UC-781 gel in sexually active HIV-uninfected women, and their male partners, and sexually abstinent HIV-infected women

Design: Single-center, phase I randomized, double-blind, controlled trial with 14 days of twice-daily product or control gel exposure

Study population: Sexually active, HIV-uninfected women at low-risk for HIV and their male partners; sexually-abstinent HIV-infected women; all age 18-45

Size: 36 sexually active HIV-uninfected women and up to 36 of their male partners; 18 sexually abstinent HIV-infected women

Treatment regimen:

Stage 1: HIV-uninfected, sexually active women and 1 male partner per woman:

12 women assigned to 0.1% UC-781 twice daily x 14 days; 12 women assigned to 0.25% UC-781 twice daily x 14 days; 12 women assigned to control gel twice daily X 14 days.

Stage 2: HIV-infected, abstinent women: 12 assigned to 0.25% UC-781 twice daily x 14 days; 6 assigned to control gel twice daily x 14 days

Study duration: Participant accrual will take 6 months. Each participant will be followed for 14 days in Stage 1 and 21 days in Stage 2. Total study duration will be 9 months.

Study Site: The Hope Clinic of Emory University, Decatur, GA

Primary Objectives:

* To assess the safety and toxicity of 0.1% UC-781 gel and 0.25% UC-781 gel administered intravaginally twice-daily for 14 days on the vulvar and cervicovaginal mucosa of sexually active HIV-uninfected women

* To assess the safety and toxicity of 0.25% UC-781 gel administered intravaginally twice-daily for 14 days on the vulvar and cervicovaginal mucosa of sexually abstinent HIV-infected women

Secondary Objectives:

* To assess the acceptability of 0.1% and 0.25% UC-781 gel administered intravaginally twice-daily for 14 days among HIV-uninfected, and the acceptability of 0.25% gel similarly administered to HIV-infected women

* To assess the effect of study product on vaginal microflora of HIV-uninfected and HIV-infected women

* To assess the systemic absorption of 0.1% and 0.25% UC-781 gel when administered intravaginally

* To assess HIV viral load in genital secretions in HIV-infected women during 0.25% UC-781 gel use

* To assess the genotypic resistance patterns of HIV in genital secretions and peripheral blood in HIV-infected women during 0.25% UC-781 gel use

* To assess the acceptability of 0.1% and 0.25% UC-781 gel in male sexual partners of HIV-uninfected women

Primary Endpoints:

The safety and toxicity of 0.1% UC-781 gel and 0.25% UC-781 gel administered intravaginally twice-daily for 14 days in sexually-active HIV-uninfected women and 0.25% UC-781 gel administered in sexually-abstinent HIV-infected women will be assessed by:

* Macroscopic and/or microscopic evidence of vulvar and/or vaginal epithelium damage, including ulceration, abrasion, severe erythema, and/or severe edema

* Macroscopic and/or microscopic evidence of cervical mucosal damage including ulceration, abrasion, severe erythema, and/or severe edema

* Symptoms of genital irritation, including burning, itching or soreness

* Laboratory evidence of grade 3 or higher toxicity for hematology, liver or renal function which cannot be attributed to another cause

Secondary Endpoints:

Acceptability of 0.1% and 0.25% UC-781 gel use by HIV-uninfected and 0.25% UC-781 gel use by HIV-infected women will be assessed by:

* Proportion of participants who at Day 14 visit indicate they would be 'somewhat unlikely' or 'very unlikely' to use 0.1% and 0.25% UC-781 gel during sexual intercourse in the future compared to control gel users

* Reported positive aspects of using 0.1% and 0.25% UC-781 gel compared to control gel users

* Reported negative aspects of using 0.1% and 0.25% UC-781 gel compared to control gel users

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
90
Inclusion Criteria

For All Females:

  • Ages 18-45 years of age

  • Able to provide written informed consent

  • Normal Pap smear at screening or documentation of such within six months prior

  • Regular monthly menses or amenorrhea due to hormonal contraceptive use

  • Agree to pelvic exam, colposcopy and biopsy (if indicated) per protocol

  • Able/willing to complete Study Diary

  • Agree to abstain from sexual intercourse for 48 hours prior to the enrollment visit

  • Agree to apply assigned study gel as required per protocol

  • Agree to abstain from the following activities from at least 48 hours prior to enrollment through the Day 14 visit:

    • Insertion of fingers/objects into the vagina
    • Receiving oral sex
    • Receiving anal sex
    • Using a diaphragm, cervical cap, female condom, or vaginal contraceptive ring
    • Using vaginal products other than the study gels
    • Participating in other vaginal microbicide or contraceptive studies

Additional Inclusion Criteria for Stage 1:

  • HIV-uninfected
  • In a monogamous sexually active relationship with one male partner throughout the study
  • Report having vaginal intercourse only with that partner at least two times per week
  • Agree to use study-provided male condoms for each act of vaginal intercourse while taking part in the study
  • Agree to inform male partner about participation

Additional Inclusion Criteria for Stage 2:

  • HIV-infected
  • Sexually abstinent or agree to abstain from sexual intercourse while taking part in the study
  • Under regular medical care for HIV management
  • CD4+ lymphocyte count > 200/mm3 for the last 6 months
  • HIV viral load > 4.0 log10 copies/ml at screening
  • Documentation of prior HIV genotype with one or more mutations conferring resistance to a non-nucleoside reverse transcriptase inhibitor (NNRTI)
  • Not currently on antiretrovirals
  • Willing to provide study staff with access to medical records related to their HIV infection

Inclusion Criteria for Male Partners:

  • Ages 18 years or older
  • Able to give written informed consent
  • Male sexual partners of HIV-uninfected women taking part in Stage 1 of the study
Exclusion Criteria

Exclusion Criteria for All Females:

  • Are post-menopausal

  • Have had a hysterectomy

  • Clinically significant chronic medical condition (other than HIV) that is considered progressive.

  • History of malignancy, with the exception of basal cell or squamous cell skin cancer

  • Pregnant or planning to become pregnant in the next three months

  • Currently breastfeeding

  • History of sensitivity or allergy to latex or any compound used in this study

  • Have received antibiotics in the 14 days prior to enrollment

  • Have used a spermicide or spermicidally lubricated condom within 7 days prior to enrollment

  • Have been using a hormonal contraceptive method for less than 3 months prior to enrollment

  • Have participated in other microbicide or contraceptive studies in the past three months

  • Grade 3 or higher liver, renal or hematology abnormality, as defined by the Table for Grading the Severity of Adult Adverse Events at screening

  • Have a positive bacterial urine culture

  • Currently have a clinically detectable genital abnormality (i.e. vulvar, vaginal, cervical and/or perineal ulcer or lesions or abnormal Pap smear)

  • In the three months prior to enrollment have had any of the following:

    • An abnormal Pap smear
    • A pregnancy
    • An abortion
    • An intrauterine device (IUD)
    • Breakthrough menstrual bleeding
    • Vaginal bleeding during or following vaginal intercourse
    • Gynecologic surgery
    • Signs consistent with a sexually transmitted disease (STD)
    • Signs of genital trauma
    • Signs of genital tract infection other than asymptomatic bacterial vaginosis (BV)
  • In the six months prior to enrollment have had any of the following:

    • History of treatment for or a diagnosis with a new STD
    • Exchanged sex for money, drugs or gifts
    • Protected (with condoms) penile-vaginal or penile-anal sexual contact with more than 4 partners
    • Unprotected (without condoms) penile-vaginal or penile-anal sexual contact with more than 1 partner
    • A male sexual partner who was diagnosed or treated for an STD (other than HIV)
    • A male sexual partner who has injected drugs
  • Are currently using, or in the last year have used intravenous drugs (except for therapeutic use), crack or other recreational drugs

  • Are currently abusing, or in the last year have abused alcohol.

  • Any other condition that, in the opinion of the investigator, might interfere with the evaluation of the study objectives

Additional Exclusion Criteria for Stage 1:

  • Unprotected (without condoms) or protected (with condoms) penile-vaginal or penile-anal sexual contact with an HIV-infected partner in the past 6 months
  • Unwilling to use study-provided male condoms while on study

Additional Exclusion Criteria for Stage 2:

  • NNRTI mutations in plasma and/or genital secretions at screening

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
3topical vaginal application of UC-781 gel-
1topical vaginal application of UC-781 gel0.25% gel
2topical vaginal application of UC-781 gel0.1% gel
Primary Outcome Measures
NameTimeMethod
Macroscopic and/or microscopic evidence of cervical, vulvar and/or vaginal epithelial damage, including ulceration, abrasion, severe erythema, and or severe edema.with two weeks of administration
Symptoms of genital irritation, including burning, itching or sorenessWith 2 weeks of administration
Laboratory evidence of grade 3 or higher toxicity for hematology, liver or renal function which cannot be attributed to another causewith 2 weeks of administration
Secondary Outcome Measures
NameTimeMethod
Acceptability of 0.1% and 0.25% UC-781 gel use by HIV-uninfected and HIV-infected womenqueried after 2 weeks of administation

Trial Locations

Locations (1)

Hope Clinic of Emory University

🇺🇸

Decatur, Georgia, United States

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