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Εffectiveness of Intrauterine Antibiotic Administration for Treatment of Chronic Endometritis

Phase 2
Recruiting
Conditions
Chronic Endometritis
Interventions
Drug: Intrauterine infusion
Drug: Oral administration
Registration Number
NCT05205993
Lead Sponsor
Genesis Athens Clinic
Brief Summary

Chronic Endometritis (CE) is related to infertility and entails a challenging management. This study investigates the treatment of off-label intrauterine antibiotic infusion either separately or combined with oral antibiotic administration, and it assesses respective performance against the gold standard treatment of oral antibiotic adminstration. Data sourced herein reports on treatment efficiency, defined as a negative diagnosis for chronic endometritis.

Detailed Description

An infectious endometrial environment caused by certain pathologies could compromise establishment of the initial interaction between the embryo and the endometrium. Chronic endometritis (CE) is a case of a persistent endometrial inflammation caused by infectious agents namely Escherichia Coli, Enterococcus faecalis, Streptococcus agalactiae, Mycoplasma, Ureaplasma, and Chlamydia. The perplexity of managing CE is attributed to its asymptomatic nature rendering a definitive diagnosis rather challenging. The list of potential symptoms is limited and vague while manifestation of the disease may be mild and may not correspond to severity. Symptoms include pain in the pelvic cavity, dispareunia, uterine bleeding, vaginal infections and cystitis, and mild gastro-intestinal discomfort. CE is described as a chronic disorder due to its long duration and persistent nature, with potentially slow progression and complex causality, severely compromising reproductive potential of patients.

The antibiotic treatments are primarily administered orally and endometrial re-examination is performed following treatment. It should be emphasized that apart from the differences in the types of antibiotics included, there are also significant discrepancies regarding the dosage of each antibiotic and the different schemes suggested by each practice. Following the antibiotic regime, endometrial receptivity is anticipated to improve. However, a significant correlation between the antibiotic therapeutic approach and a positive results in IVF has failed to be established in literature, indicating that oral antibiotic administration is unseccessful in improving IVF outcome.

A pilot study published by the investigators indicated that combination of intrauterine infusion of antibiotics and oral administration can be more effective in treating CE than solely oral administration. However, it has not been evaluated if sole intrauterine infusion of antibiotics can be of similar effectiveness when compared to the combined regime including intrauterine infusion and oral andministration, or when compared to the gold standard treatment including only oral administration of antibiotics.

Recruitment & Eligibility

Status
RECRUITING
Sex
Female
Target Recruitment
90
Inclusion Criteria
  • Positive diagnosis of CE following hysteroscopy and endometrial biopsy (positive diagnosis is required by both evaluations)
  • Signed Informed Consent
  • 18.5 < BMI < 30
Exclusion Criteria
  • Diagnosed endometriosis or other related Pelvic Inflammatory Disorder (PID)
  • Current or previous cancer diagnosis
  • Auto-immune or genetic disorders
  • Menstrual cycle disorders

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Intrauterine infusion of antibioticsIntrauterine infusionThirty women will receive only intrauterine infusion of antibiotics for 30 days.
Combination of intrauterine infusion and oral administration of antibioticsIntrauterine infusionThirty women will receive a combination of intrauterine infusion and oral administration of antibiotics for 30 days.
Oral administration of antibioticsOral administrationThirty women will receive only oral administration of antibiotics for 30 days.
Combination of intrauterine infusion and oral administration of antibioticsOral administrationThirty women will receive a combination of intrauterine infusion and oral administration of antibiotics for 30 days.
Primary Outcome Measures
NameTimeMethod
Negative diagnosis of CE following hysteroscopy and endometrial biopsyNegative diagnosis of CE will be assessed in one month -immidiately after treatment completion-.

All participants will be subjected to hysteroscopy and endometrial biopsy following completion of treatment. Regarding hysteroscopy the presence of endometritis will be evaluated according to the published unified diagnostic criteria for CE. The endometrial biopsy will be performed employing a pipelle. To minimize contamination risk, following the placement of a vaginal speculum and cleaning external uterine ostium with an iodine solution, the pipelle will be inserted under visual control into the uterine cavity ensuring avoidance of any contact with vaginal walls. Endometrial samples will be diluted into 2 ml of saline for histological analysis. For histological analysis the endometrium samples will be fixated employing neutral formalin and paraffin. To stain the microsections, hematoxyline and eosin will be engaged. CD138 staining will be performed and positivity will indicate a CE diagnosis (\>1 plasma cell per 10HPFs). A negative CE diagnosis should be confirmed by both assessments

Secondary Outcome Measures
NameTimeMethod
Number of participants with treatment-related adverse events as assessed by CTCAE v6.0Side-effects will be monitored for one month from treatment initiation which is until completion of treatment

Number of reports of side-effects according to CTCAE v6.0 per treated patients

Trial Locations

Locations (1)

Genesis AC

🇬🇷

Athens, Greece

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