Proteomic Analysis of the Peripheral-Blood Mononuclear Cells Response After a High-Fat, High-Carbohydrate Meal With or Without Orange Juice in Healthy Subjects
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 12
- 主要终点
- Change in protein expression at mononuclear cells of healthy individuals before and after the high fat meal using TMT labeling and mass spectrometry
研究概览
简要总结
A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers. The present study will assess the effect of a single HFHC meal taken with water, orange juice or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals. Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals.
详细描述
BACKGROUND:
A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers.
OBJECTIVES:
To study the effect of a single HFHC meal taken with water, orange juice (500ml) or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals.
DESIGN AND METHODOLOGY:
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •normal weight
- •non-vegetarian
排除标准
- •use of medication
- •alcohol consumption,
- •intense physical exercise
- •pregnant or lactating
- •overweight
- •drug abuse
- •any disease such as diabetes, inflammatory conditions.
研究组 & 干预措施
Orange juice
The subjects will ingest the HFHC meal with 500mL of orange juice.
干预措施: Orange juice (Other)
Water with glucose
The subjects will ingest the HFHC meal with 500mL of water with glucose (isocaloric control of the orange juice).
干预措施: Water with glucose (Other)
Water
The subjects will ingest the HFHC meal with 500mL of water.
干预措施: Water (Other)
结局指标
主要结局
Change in protein expression at mononuclear cells of healthy individuals before and after the high fat meal using TMT labeling and mass spectrometry
时间窗: before the meal, 1h, 3h and 5h after the meal
次要结局
- Blood glucose(5h after the meal)
- Insulin(5h after the meal)
- Lipid profile(5h after the meal)
- Blood glucose(before the meal)
- Blood glucose(1h after the meal)
- Blood glucose(3h after the meal)
- Insulin(before the meal)
- Insulin(1h after the meal)
- Insulin(3h after the meal)
- Lipid profile(before the meal)
- Lipid profile(1h after the meal)
- Lipid profile(3h after the meal)
研究者
Daniela Chaves
PhD
University of Sao Paulo