Proteomic Analysis of Mononuclear Cells After a High-Fat, High-Carbohydrate Meal With or Without Orange Juice

Not Applicable

Completed

• Conditions: Inflammation
• Interventions: Other: Orange juice; Other: Water; Other: Water with glucose

• Registration Number: NCT02587507
• Lead Sponsor: University of Sao Paulo

Brief Summary

A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers. The present study will assess the effect of a single HFHC meal taken with water, orange juice or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals. Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals.

Detailed Description

BACKGROUND:

A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers.

OBJECTIVES:

To study the effect of a single HFHC meal taken with water, orange juice (500ml) or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals.

DESIGN AND METHODOLOGY:

Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals. Blood glucose, insulin and lipid profile were measured. Also, proteome analysis using TMT labeling was performed on peripheral-blood mononuclear cells collected before and at times 3 and 5h after the meal.

Study Timeline

• Study Start: 2015-06
• Primary Completion: 2015-09
• Study Completion: 2015-10
• Study First Submitted: 2015-10-22
• Study First Submitted QC: 2015-10-23
• Study First Posted: 2015-10-27
• Status Verified: 2016-07
• Last Update Submitted: 2016-07-27
• Last Update Posted: 2016-07-29

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 12

Inclusion Criteria

• healthy
• normal weight
• non-vegetarian

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Exclusion Criteria

• use of medication
• alcohol consumption,
• intense physical exercise
• pregnant or lactating
• overweight
• obese
• drug abuse
• any disease such as diabetes, inflammatory conditions.

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Orange juice	Orange juice	The subjects will ingest the HFHC meal with 500mL of orange juice.
Water	Water	The subjects will ingest the HFHC meal with 500mL of water.
Water with glucose	Water with glucose	The subjects will ingest the HFHC meal with 500mL of water with glucose (isocaloric control of the orange juice).

Primary Outcome Measures

Name	Time	Method
Change in protein expression at mononuclear cells of healthy individuals before and after the high fat meal using TMT labeling and mass spectrometry	before the meal, 1h, 3h and 5h after the meal

Secondary Outcome Measures

Name	Time	Method
Blood glucose	5h after the meal
Insulin	5h after the meal
Lipid profile	5h after the meal	total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides