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Effects of Advanced Glycation Endproducts on Satiety and Inflammation

Not Applicable
Completed
Conditions
Obesity
Type 2 Diabetes
Insulin Resistance
Cardiovascular Disease
Interventions
Other: Dietary meal intervention
Registration Number
NCT01434407
Lead Sponsor
University of Copenhagen
Brief Summary

The purpose of the meal study is to investigate acute effects on satiety and inflammation of advanced glycation endproducts (AGE) in healthy overweight subjects. The AGE content of the meal is affected by food preparation methods: frying/grilling versus boiling/steaming.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
19
Inclusion Criteria
  • Healthy males and females between 20 and 50 years old
  • BMI 25-40
  • Waist circumference above 88 cm for women and above 102 cm for men
Exclusion Criteria
  • Exercise training above 8 hour/week
  • Smoking
  • Pregnant or breast-feeding women
  • Post-menstrual women
  • Regular use of medicine (except contraceptive pills)
  • Previous gastric bypass surgery
  • Donation of blood within the last 3 months
  • Involvement in other clinical trials
  • Allergic to paraaminobenzoic acid

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Low AGE mealDietary meal interventionTest meal prepared by boiling/steaming the food
High AGE mealDietary meal interventionTest meal prepared by frying/grilling the food
Primary Outcome Measures
NameTimeMethod
Appetite sensationEvery 30 min up to 5 hours after test meal

Appetite sensation measured by visual analogue scales

Plasma ghrelinContinuous measurement up to 5 hours after test meal
Plasma GLP-1Continuous measurements up to 5 hours after test meal
Plasma PYYContinuous measurements up to 5 hours after test meal
Plasma insulinContinuous measurements up to 5 hours after test meal
Plasma glucoseContinuous measurements up to 5 hours after test meal
Urine AGEFrom 24 hour before to 48 hour after test meal
Secondary Outcome Measures
NameTimeMethod
IL-6Continuous measurements up to 5 hours after test meal
TNF-alphaContinuous measurements up to 5 hours after test meal
MIFContinuous measurements up to 5 hours after test meal
IL-1 betaContinuous measurements up to 5 hours after test meal
Plasma triglyceridesContinuous measurements up to 5 hours after test meal
Expression of genes related to AGEContinuous measurements up to 24 hours after test meal
CRPContinuous measurements up to 5 hours after test meal
VCAMContinuous measurements up to 5 hours after test meal
ICAMContinuous measurements up to 5 hours after test meal
Plasma AGEContinuous measurements up to 48 hours after test meal
Urine isoprostanesFrom 24 hour before to 24 hours after test meal

Trial Locations

Locations (1)

Department of Human Nutrition, University of Copenhagen

🇩🇰

Copenhagen, Frederiksberg C, Denmark

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