The assessment of long-term in vivo muscle protein synthesis rates in ICU patients using deuterated water methodology
Recruiting
- Conditions
- Muscle wastingProtein synthesis100040181003700810028393
- Registration Number
- NL-OMON55410
- Lead Sponsor
- Medisch Universitair Ziekenhuis Maastricht
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 12
Inclusion Criteria
1) Age >18 <90 years
2) Arterial line (any location) in situ
3) Enteral feeding tube in situ
4) Expected ICU stay >5 days
Exclusion Criteria
1) Coagulation disturbances (not including use of anti-coagulants in
prophylactic dosages)
2) Contraindication to enteral infusion (e.g. due to GI-tract perforation)
3) Rhabdomyolysis
4) Renal dysfunction defined as a serum creatinine >171 *mol/L or a urine
output of less than 500 ml/last 24 hours
5) Patients requiring chronic veno-venous hemofiltration
6) Patients on any form of extracorporeal life support (ECMO/ELS)
7) Pregnant patients or lactating with the intent to breastfeed
8) Previous participation in a 2H amino acid tracer study within the last year
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Primary study parameters are the fractional rates of muscle protein synthesis<br /><br>(%/day) using muscle protein-bound 2H-alanine enrichments, plasma 2H-alanine<br /><br>enrichments and intramuscular free 2H-alanine enrichment. </p><br>
- Secondary Outcome Measures
Name Time Method <p>Secondary study parameters include: body water 2H2O enrichment, plasma<br /><br>muscle-specific protein synthesis rates (virtual biopsy), transcriptional<br /><br>changes in genes involved in muscle protein synthesis and breakdown and plasma<br /><br>amino acid concentrations. </p><br>