Prolonged muscle protein synthetic response to the ingestion of a large amount of protei

Completed

Conditions: muscle protein synthesis
Spieropbouw na inspanning
building muscle protein

Registration Number: NL-OMON48402

Lead Sponsor: niversiteit Maastricht

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 45

Inclusion Criteria

- Males - Aged between 18-40 years - Healthy - 18.5 * BMI * 30 kg/m2

Exclusion Criteria

- Smoking - Sports/exercise > 4 sessions/week - Lactose intolerant or allergies to milk protein - A history of neuromuscular problems - Use of anticoagulation medication - Recent (<9 mo) participation in amino acid tracer (L-[ring-2H5-phenylalanine, L-[ring-2H2]-tyrosine, and [1-13C]-leucine infusion) studies - Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription acne medications) - Strict vegetarian - Injury or condition that would limit the participant from performing the resistance exercise

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Myofibrillar protein synthesis rates over the full assessment period (12 h)</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>- Myofibrillar protein synthesis rates assessed over 3 periods (0-4, 4-8 and<br /><br>8-12 hours)<br /><br>- 1-13C-phenylalanine incorporation into muscle protein<br /><br>- Plasma amino acid concentrations & enrichments<br /><br>- Plasma glucose & insulin concentrations<br /><br>- Whole-body protein metabolism (synthesis, breakdown, oxidation, balance)<br /><br>- Whole-body protein kinetics (total rate of appearance, rate of endogenous<br /><br>appearance, rate of exogenous appearance, rate of disappearance)</p><br>