Postprandial muscle protein synthesis following wheat protein ingestion in vivo in humans

Completed

Conditions: age-related muscle loss
sarcopenia
10037008
10028302

Registration Number: NL-OMON38800

Lead Sponsor: Medisch Universitair Ziekenhuis Maastricht

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 60

Inclusion Criteria

* Healthy males
* Age between 65 and 80 y
* BMI between 18.5 and 30 kg/m2

Exclusion Criteria

Wheat allergy
Celiac disease
Lactose intolerance
Smoking
Diabetes
Diagnosed GI tract diseases
Arthritic conditions
A history of neuromuscular problems
Any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
Use of anticoagulants
Participation in exercise program
Hypertension, high blood pressure that is above 140/90 mmHg.

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The main study endpoint is muscle protein synthesis (MPS) rates. In order to<br /><br>determine the MPS, the following parameters will be measured:<br /><br>* Muscle protein-bound L-[U-13C]-phenylalanine enrichment (expressed as MPE)<br /><br>* Muscle free (intracellular) L-[U-13C]-phenylalanine enrichment (expressed as<br /><br>MPE)<br /><br>* Plasma L-[U-13C]-phenylalanine enrichment (expressed as MPE)</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Secondary endpoints include whole-body protein metabolism (synthesis,<br /><br>breakdown, oxidation, and net balance). Therefore, the following parameters<br /><br>will be measured:<br /><br>* Plasma phenylalanine and tyrosine concentration (expressed as mol/L)<br /><br> Plasma enrichments (in MPE) of:<br /><br>o L-[U-13C]-phenylalanine<br /><br>o L-[U-13C]-tyrosine<br /><br>o L-[3,5-2H2]-tyrosine<br /><br>Other study parameters include plasma leucine, glucose, and insulin<br /><br>concentrations, age, body weight, body length, BMI, body composition, blood<br /><br>pressure, and leg volume. </p><br>