AV study.
Recruiting
- Conditions
- oss of muscle mass in healthy aging (sarcopenia)
- Registration Number
- NL-OMON22412
- Lead Sponsor
- Maastricht University
- Brief Summary
/A
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 48
Inclusion Criteria
1. Healthy untrained men;
2. Age 70-85 years or age 18-30 years;
Exclusion Criteria
1. Cardiac abnormalities;
2. Coagulation disorders;
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method The main study endpoint is the muscle protein synthesis rate, expressed as fractional synthetic rate (FSR). In order to determine the FSR, the following parameters will be measured in blood and muscle tissue:<br /><br>1. Plasma and muscle free phenylalanine concentration (expressed as μmol/L);<br /><br>2. Plasma enrichment of L-[ ring-2H5]phenylalanine (expressed as mole percent excess (MPE));<br /><br>3. Muscle protein bound enrichment of L-[ ring-2H5]phenylalanine (expressed as MPE);<br /><br>4. L-[ ring-2H5]phenylalanine enrichment of the muscle free amino acid pool (expressed as MPE).
- Secondary Outcome Measures
Name Time Method Secondary endpoints include whole body protein turnover, protein digestion and absorption kinetics and microvascular perfusion. The following parameters will be calculated:<br /><br>1. Exogenous phenylalanine rate of appearance and plasma availability of phenylalanine;<br /><br>2. Total rate of phenylalanine appearance and disappearance (= protein turnover);<br /><br>3. Endogenous phenylalanine rate of appearance (=protein breakdown);<br /><br>4. Femoral blood flow using Doppler ultrasound expressed as L/min;<br /><br>5. Leg blood flow using ICG dye dilution expressed as mL/min/100mL leg;<br /><br>6. Sublingual microvascular perfusion measured using the SDF camera expressed as: Proportion of perfused vessels (PPV; %), perfused vessel density (PVD; n/mm) and Microcirculatory flow index (MFI).