Time Course of Postprandial Protein Metabolism

Not Applicable

Completed

• Conditions: Protein Metabolism; Muscle Protein Synthesis
• Interventions: Dietary Supplement: Milk Protein Concentrate

• Registration Number: NCT04121689
• Lead Sponsor: University of Illinois at Urbana-Champaign

Brief Summary

The anabolic action of 'fast' whey protein on the regulation of postprandial muscle protein synthesis has been established to be short-lived in healthy young adults. Our aim was assess the time course of anabolic signaling events and stimulation of muscle protein synthesis rates (MPS) after ingestion of a food source that represents a more typical meal-induced pattern of aminoacidemia, namely milk protein concentrate, in healthy young males.

Detailed Description

Not available

Study Timeline

• Study Start: 2014-10-01
• Primary Completion: 2014-11-01
• Status Verified: 2019-08
• Study Completion: 2019-08-26
• Study First Submitted: 2019-09-03
• Study First Submitted QC: 2019-10-07
• Last Update Submitted: 2019-10-07
• Study First Posted: 2019-10-10
• Last Update Posted: 2019-10-10

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 7

Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Milk Protein Concentrate	Milk Protein Concentrate	Seven young men (age: 22±1 y) will undergo repeated blood and biopsy sampling during primed continuous L-\[ring-2H5\]phenylalanine and L-\[1-13C\]leucine tracer infusions, and ingested 38 g of L-\[1-13C\]phenylalanine- and L-\[1-13C\]leucine-labeled milk protein concentrate

Primary Outcome Measures

Name	Time	Method
Fractional synthesis rates of myofibrillar protein	8 hours	Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hours, and every 1-2 hours during the 5 hours after protein ingestion. This will allow us assess the timecourse of muscle protein synthesis after eating.

Secondary Outcome Measures

Name	Time	Method
Exogenous rate of phenylalanine appearance	5 hours	Dietary derived phenylalanine availability will be measured during the 5 h postprandial phase to determine its relationship with the fractional synthesis rates of myofibrillar protein and phosphorylation of muscle anabolic signaling
Phosphorylation of muscle anabolic signaling	8 hours	Phosphorylation of anabolic signaling pathways (mTORC1, AKT, and P70S6K) involved in the stimulation of myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hours, and every 1-2 hours during the 5 hours after protein ingestion.

Trial Locations

Locations (1)

Freer Hall; 🇺🇸 Urbana, Illinois, United States