Protein synthesis rates in muscle tissue in vivo in patients with mitochondrial myopathy after high intensity interval exercise training

Conditions: metabolic diseases
metabolic myopathy
10028302

Registration Number: NL-OMON51490

Lead Sponsor: Radboud Universitair Medisch Centrum

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Pending

Sex: Not specified

Target Recruitment: 16

Inclusion Criteria

- Age >= 18 years
- m.3243A>G point mutation or single, large scale mtDNA deletion

Exclusion Criteria

* insufficient mastery of the Dutch language
* pregnancy
* severe cardiopulmonary disease (chest pain, heart failure, arrhythmia,
pacemaker, cardiac surgery, severe exertional dyspnea, emphysema). If cardiac
involvement is present, inclusion will be evaluated by one of the cardiologists
with expertise in mitochondrial cardiomyopathy.
* coagulopathy (anti-platelet therapy is allowed)
* cognitive dysfunction
* not able to perform the high intensity training protocol

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Primary study parameters include mitochondrial and myofibrillar protein<br /><br>synthesis rates of vastus lateralis muscle, calculated from the increase in<br /><br>enriched deuterized alanine in muscle tissue. The trained muscle will be<br /><br>compared with the untrained muscle after 14 days of aerobic exercise.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Secondary study parameters are muscle tissue amino acid composition and<br /><br>nitrogen content. Also mitochondrial respiration of muscle tissue from the<br /><br>trained and untrained leg will be compared to baseline tissue using the<br /><br>Oroboros technique. </p><br>