Regeneration of Posterior Cruciate Ligament Injury Using Hypoxia Conditioned Allogenic Adipose Mesenchymal Stem Cell (AdMSC) and Ligament Derived Condition Medium (Secretome)
试验速览
- 阶段
- 早期 1 期
- 发起方
- 入组人数
- 16
- 试验地点
- 1
- 主要终点
- Thrombosite of Growth Factor - beta 1
研究概览
简要总结
Transplantation of Allogenic Adipose Mesenchimal Stem cell in hypoxic cultur condition with Ligament- derived conitioned medium can enhance regeneration of posterior cruciate ligament rupture
详细描述
this study is in vivo study to prove effect of transplantation of Allogenic Adipose Mesenchimal Stem cell in hypoxic cultur condition with Ligament- derived conitioned medium in regeneration of posterior cruciate ligament rupture
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 2 Months 至 12 Months（Child）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •white male new zealand rabbit
- •weight 2500 - 3000 gram
排除标准
- •have no disease
研究组 & 干预措施
control group
rabbit with posterior cruciate ligament rupture and treated with fibrin glue (sacffold)
干预措施: adypose mesenchymal conditioned medium and ligament derived conditioned medium (Biological)
conditioned medium group
rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with ligament derived conditioned medium
干预措施: adypose mesenchymal conditioned medium and ligament derived conditioned medium (Biological)
stem cells group
rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with adypose mesenchymal stem cells in hypoxic culture condition
干预措施: adypose mesenchymal conditioned medium and ligament derived conditioned medium (Biological)
composite group
rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with adypose mesenchymal stem cells in hypoxic culture condition and ligament derived conditioned medium
干预措施: adypose mesenchymal conditioned medium and ligament derived conditioned medium (Biological)
结局指标
主要结局
Thrombosite of Growth Factor - beta 1
时间窗: 6 weeks
measurement value of Thrombosite Growth Factor - beta 1
basic fibroblast of growth factor
时间窗: 6 weeks
measurement value of basic fibroblast of growth factor
tensile strength of posterior cruciate ligament
时间窗: 6 weeks
measurement of tensile strength of posterior cruciate ligament
次要结局
未报告次要终点
研究者
Sholahuddin Rhatomy
Director, Head of Orthopaedic Department,faculty of medicine,public health and nursing,universitas gadjah mada, Sholahuddin Rhatomy,MD
Gadjah Mada University