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A Dose Escalation and Expansion Study Evaluating the Safety, Tolerability, Pharmacokinetics, and Pharmacodynamics of RO7283420.

Phase 1
Completed
Conditions
Acute Myeloid Leukemia
Interventions
Registration Number
NCT04580121
Lead Sponsor
Hoffmann-La Roche
Brief Summary

This open-label, entry-into-human (EIH) study will evaluate the safety, tolerability, pharmacokinetics (PK), and pharmacodynamics of RO7283420. Escalating doses of RO7283420 will be administered to participants with Acute Myeloid Leukemia (AML) in order to determine the maximum tolerated dose (MTD) and/or recommended Phase II dose (RP2D).

Detailed Description

The study will include AML participants with measurable disease, for whom standard-of-care (SOC) is not available. Two Groups of AML participants will be included in this study:

* Group I participants will have hematologic relapse/refractory disease defined as participants not in complete remission (CR) or complete remission with incomplete hematologic recovery (CRi).

* Group II participants will have molecular relapse/persistent disease (participants with a CR or CRi, and a positive MRD based on local multi-parameter flow cytometry (MFC) or molecular assessment).

The study consists of three parts:

* Part A (single-participant dose escalation cohorts) - single participants from Group I will receive increment-based escalating doses until a Grade \>=2 AE related to RO7283420 or a clear pharmacodynamic effect

* Part B (multiple-participant dose escalation cohorts) - multiple-participant cohorts of \>=3 participants will be enrolled for dose escalation for Group I and Group II independently.

* Part C (dose expansion) - participants will receive the respective identified RP2D for that group.

The treatment period for each participant will be up to 7 months with a maximum number of cycles depending on the dosing frequency the participant receives. Each participant will receive up to 6, 9, and 18 cycles of treatment with RO7283420, when treated with Q3W, every-2-weeks (Q2W), or once-a-week (QW) dosing regimens, respectively. Additional 3, 5, or 9 cycles may be administered for the Q3W, Q2W, and QW dosing regimens, respectively, in case the participants have achieved at least partial remission (PR).

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
59
Inclusion Criteria
  • With confirmed diagnosis of primary or secondary AML according to WHO classification 2016, with measurable disease. Eligible participants need to have received standard-of-care (SOC) and have no other SOC options available Participants who are not willing to receive SOC will be not eligible. Two groups of participants (Group I - hematologic relapsed/refractory and Group II - molecular relapsed/refractory) will be included
  • Participants who have received hematopoietic stem cell transplant (HSCT) must have the HSCT performed ≥ 90 days prior to the first dose of RO7283420 on Cycle 1 Day 1, having demonstrated hematological engraftment and do not have an active Graft versus Host Disease, not requiring immunosuppressive treatment (including but not limited to cyclosporine, tacrolimus, sirolimus, and mycophenolate), which must be stopped at least 28 days prior to the first dose of RO7283420 on Cycle 1 Day 1
  • Eastern Cooperative Oncology Group (ECOG) performance status 0-2
  • Peripheral blast counts =< 20,000/mm3 on Cycle 1 Day 1 prior to the first dosing
  • Confirmed genotype of HLA-A*02
  • Adequate renal (a creatinine clearance of >=50 mL/min as calculated according to the Cockroft-Gault formula) and adequate liver test results
  • Male or female participants agree to use contraception and the abstinence requirements to prevent exposure of an embryo to the study treatment
Exclusion Criteria
  • Acute promyelocytic leukemia (APL)
  • Core Binding Factor (CBF)-AML Note: participants with r/r CBF-AML after at least 2 salvage attempts can be enrolled into the study
  • Group II only: participants with normal karyotype and a favorable molecular profile according to ELN guideline 2017
  • Participants with active bacterial, fungal or viral infection considered by the Investigator to be clinically uncontrolled or of unacceptable risk upon the induction of neutropenia (i.e. participants who are or should be on antimicrobial agents for the treatment of active infection)
  • Grade >= 2 glomerular proteinuria at screening or on Cycle 1 Day 1 prior to the first dosing.
  • Another primary malignancy (other than AML) that requires active therapy. Adjuvant hormonal therapy is allowed
  • Clinical evidence or history of central nervous system (CNS) leukemia
  • Presence of extramedullary disease at screening
  • Current or past history of CNS disease, such as stroke, CNS inflammation, epilepsy, CNS vasculitis, or neurodegenerative disease
  • Participants who have a history of clinically significant liver disease, including liver cirrhosis (e.g. Child-Pugh class B and C) or participants who have a history of active or chronic infectious hepatitis unless serology demonstrates clearance of infection
  • Participants who might refuse to receive blood products and/or have known hypersensitivity to any of the components of RO7283420, tocilizumab, or dasatinib

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
Part A: Single Participant Dose EscalationParacetamol/acetaminophenParticipants from Group I will receive escalating doses of RO7283420, once every 3 weeks (Q3W) starting on Cycle 1, Day 1 (C1D1) for up to 6 cycles with a starting dose of 0.15mg.
Part C: Dose ExpansionParacetamol/acetaminophenParticipants will receive the respective RP2D for Group I and Group II.
Part B: Multiple Participant Dose EscalationParacetamol/acetaminophenMultiple-participant cohorts of \>= 3 participants will be enrolled for dose escalation for Group I and Group II independently. Participants will be administered a starting dose of 0.15 mg or highest dose administered in Part A. Each participant will receive up to 6, 9, and 18 cycles of treatment with RO7283420, when treated with Q3W, every-2-weeks (Q2W), or once-a-week (QW) dosing regimens, respectively to determine the maximum tolerated dose (MTD) and recommended Phase 2 dose (RP2D). Additionally, step-up dosing regimens with more frequent administrations of RO7283420 during cycle 1 will be evaluated.
Part C: Dose ExpansionRO7283420Participants will receive the respective RP2D for Group I and Group II.
Part A: Single Participant Dose EscalationRO7283420Participants from Group I will receive escalating doses of RO7283420, once every 3 weeks (Q3W) starting on Cycle 1, Day 1 (C1D1) for up to 6 cycles with a starting dose of 0.15mg.
Part B: Multiple Participant Dose EscalationRO7283420Multiple-participant cohorts of \>= 3 participants will be enrolled for dose escalation for Group I and Group II independently. Participants will be administered a starting dose of 0.15 mg or highest dose administered in Part A. Each participant will receive up to 6, 9, and 18 cycles of treatment with RO7283420, when treated with Q3W, every-2-weeks (Q2W), or once-a-week (QW) dosing regimens, respectively to determine the maximum tolerated dose (MTD) and recommended Phase 2 dose (RP2D). Additionally, step-up dosing regimens with more frequent administrations of RO7283420 during cycle 1 will be evaluated.
Part A: Single Participant Dose EscalationTocilizumabParticipants from Group I will receive escalating doses of RO7283420, once every 3 weeks (Q3W) starting on Cycle 1, Day 1 (C1D1) for up to 6 cycles with a starting dose of 0.15mg.
Part A: Single Participant Dose EscalationDasatinibParticipants from Group I will receive escalating doses of RO7283420, once every 3 weeks (Q3W) starting on Cycle 1, Day 1 (C1D1) for up to 6 cycles with a starting dose of 0.15mg.
Part A: Single Participant Dose EscalationDexamethasoneParticipants from Group I will receive escalating doses of RO7283420, once every 3 weeks (Q3W) starting on Cycle 1, Day 1 (C1D1) for up to 6 cycles with a starting dose of 0.15mg.
Part C: Dose ExpansionDiphenhydramineParticipants will receive the respective RP2D for Group I and Group II.
Part A: Single Participant Dose EscalationDiphenhydramineParticipants from Group I will receive escalating doses of RO7283420, once every 3 weeks (Q3W) starting on Cycle 1, Day 1 (C1D1) for up to 6 cycles with a starting dose of 0.15mg.
Part B: Multiple Participant Dose EscalationDexamethasoneMultiple-participant cohorts of \>= 3 participants will be enrolled for dose escalation for Group I and Group II independently. Participants will be administered a starting dose of 0.15 mg or highest dose administered in Part A. Each participant will receive up to 6, 9, and 18 cycles of treatment with RO7283420, when treated with Q3W, every-2-weeks (Q2W), or once-a-week (QW) dosing regimens, respectively to determine the maximum tolerated dose (MTD) and recommended Phase 2 dose (RP2D). Additionally, step-up dosing regimens with more frequent administrations of RO7283420 during cycle 1 will be evaluated.
Part B: Multiple Participant Dose EscalationTocilizumabMultiple-participant cohorts of \>= 3 participants will be enrolled for dose escalation for Group I and Group II independently. Participants will be administered a starting dose of 0.15 mg or highest dose administered in Part A. Each participant will receive up to 6, 9, and 18 cycles of treatment with RO7283420, when treated with Q3W, every-2-weeks (Q2W), or once-a-week (QW) dosing regimens, respectively to determine the maximum tolerated dose (MTD) and recommended Phase 2 dose (RP2D). Additionally, step-up dosing regimens with more frequent administrations of RO7283420 during cycle 1 will be evaluated.
Part B: Multiple Participant Dose EscalationDasatinibMultiple-participant cohorts of \>= 3 participants will be enrolled for dose escalation for Group I and Group II independently. Participants will be administered a starting dose of 0.15 mg or highest dose administered in Part A. Each participant will receive up to 6, 9, and 18 cycles of treatment with RO7283420, when treated with Q3W, every-2-weeks (Q2W), or once-a-week (QW) dosing regimens, respectively to determine the maximum tolerated dose (MTD) and recommended Phase 2 dose (RP2D). Additionally, step-up dosing regimens with more frequent administrations of RO7283420 during cycle 1 will be evaluated.
Part B: Multiple Participant Dose EscalationDiphenhydramineMultiple-participant cohorts of \>= 3 participants will be enrolled for dose escalation for Group I and Group II independently. Participants will be administered a starting dose of 0.15 mg or highest dose administered in Part A. Each participant will receive up to 6, 9, and 18 cycles of treatment with RO7283420, when treated with Q3W, every-2-weeks (Q2W), or once-a-week (QW) dosing regimens, respectively to determine the maximum tolerated dose (MTD) and recommended Phase 2 dose (RP2D). Additionally, step-up dosing regimens with more frequent administrations of RO7283420 during cycle 1 will be evaluated.
Part C: Dose ExpansionTocilizumabParticipants will receive the respective RP2D for Group I and Group II.
Part C: Dose ExpansionDasatinibParticipants will receive the respective RP2D for Group I and Group II.
Part C: Dose ExpansionDexamethasoneParticipants will receive the respective RP2D for Group I and Group II.
Primary Outcome Measures
NameTimeMethod
Percentage of Participants with Adverse Events (AEs)From baseline up to 9 months
Percentage of Participants with Dose-Limiting Toxicities (DLTs)From baseline up to 28 days
Recommended Phase II Dose (RP2D)From baseline up to 7 months
Secondary Outcome Measures
NameTimeMethod
Event-free Survival (EFS)From baseline to the time to progression, relapse, death from any cause, or start of a new treatment (up to approximately 4 years)
Duration of Response (DoR)From first occurrence of a documented response until the time of documented relapse, disease progression or death from any cause, whichever occurs first (up to approximately 4 years)
Time to Hematological Relapse (Group II Only)From baseline until the time of documented hematological relapse
Early Mortality RateFrom baseline to Day 30, and to Day 60
Maximum Reduction (%) from Baseline in Blast Count in Peripheral Blood and/or Bone Marrow (Group I Dose Escalation Cohorts only)From baseline up to 7 months
Percentage of Participants who Achieve a ResponseFrom baseline up to approximately 4 years

Defined by ELN 2017 recommendations, i.e., complete remission (CR), complete remission with incomplete hematologic recovery (CRi), complete remission with absence of measurable residual disease (CRMRD-), and partial remission (PR).

Transfusion IndependenceFrom baseline up to 7 months
Clearance (Cl) of RO7283420Day 1, 2, 3, 8, 15 of Cycle 1 (each cycle is 21 days); Day 1, 2, 3, 8 of Cycle 2 only in case of triple step-up dosing; Day 1 of Cycle 2-9 (Q3W), 2-14 (Q2W), 2-27 (QW); end of treatment visit.
Incidence and Titer of Anti-drug Antibodies (ADA) against RO7283420Day 1, 8, 15 of Cycle 1 (each cycle is 21 days); Day 1 and 8 of Cycle 2, Day 1 of Cycle 3-9 (Q3W), 3-14 (Q2W), 3-27 (QW); at end of treatment visit
Progression-free Survival (PFS)From Cycle 1 Day 1 to the first occurrence of documented disease progression, or death from any cause, whichever occurs first (up to approximately 4 years)
Number of MRD (Measurable Residual Disease) Negative Participants over time According to Local MRD AssessmentFrom baseline up to 7 months
Area Under the Curve (AUC) of RO7283420Day 1, 2, 3, 8, 15 of Cycle 1 (each cycle is 21 days); Day 1, 2, 3, 8 of Cycle 2 only in case of triple step-up dosing; Day 1 of Cycle 2-9 (Q3W), 2-14 (Q2W), 2-27 (QW); end of treatment visit.
Maximum Concentration (Cmax) of RO7283420Day 1, 2, 3, 8, 15 of Cycle 1 (each cycle is 21 days); Day 1, 2, 3, 8 of Cycle 2 only in case of triple step-up dosing; Day 1 of Cycle 2-9 (Q3W), 2-14 (Q2W), 2-27 (QW); end of treatment visit.
Minimum Concentration (Cmin) of RO7283420Day 1, 2, 3, 8, 15 of Cycle 1 (each cycle is 21 days); Day 1, 2, 3, 8 of Cycle 2 only in case of triple step-up dosing; Day 1 of Cycle 2-9 (Q3W), 2-14 (Q2W), 2-27 (QW); end of treatment visit.
Volume (V) of RO7283420Day 1, 2, 3, 8, 15 of Cycle 1 (each cycle is 21 days); Day 1, 2, 3, 8 of Cycle 2 only in case of triple step-up dosing; Day 1 of Cycle 2-9 (Q3W), 2-14 (Q2W), 2-27 (QW); end of treatment visit.
Half-life (T1/2) of RO7283420Day 1, 2, 3, 8, 15 of Cycle 1 (each cycle is 21 days); Day 1, 2, 3, 8 of Cycle 2 only in case of triple step-up dosing; Day 1 of Cycle 2-9 (Q3W), 2-14 (Q2W), 2-27 (QW); end of treatment visit.

Trial Locations

Locations (23)

Ospedale Santa Chiara; Unita Operativa Di Ematologia

🇮🇹

Pisa, Toscana, Italy

UC Davis Comprehensive Cancer Center

🇺🇸

Sacramento, California, United States

Rigshospitalet; Hæmatologisk Klinik, Klinisk Afprøvnings Team KAT

🇩🇰

København Ø, Denmark

The University of Texas MD Anderson Cancer Center

🇺🇸

Houston, Texas, United States

Princess Margaret Cancer Center

🇨🇦

Toronto, Ontario, Canada

Hospital Universitari Vall d'Hebron; Servicio de Hematologia

🇪🇸

Barcelona, Spain

Institut Catala d?Oncologia Hospital Germans Trias i Pujol

🇪🇸

Badalona, Barcelona, Spain

Hospital Univ. 12 de Octubre; Servicio de Hematologia

🇪🇸

Madrid, Spain

Hospital Clínic i Provincial; Servicio de Hematología y Oncología

🇪🇸

Barcelona, Spain

Hospital Universitario la Fe; Servicio de Hematologia

🇪🇸

Valencia, Spain

China Medical University Hospital; Oncology and Hematology

🇨🇳

Taichung, Taiwan

National Cheng Kung University Hospital; Oncology

🇨🇳

Tainan, Taiwan

Churchill Hospital

🇬🇧

Oxford, United Kingdom

Royal Marsden NHS Foundation Trust

🇬🇧

Sutton, United Kingdom

Hopital De Haut Leveque; Hematologie Clinique

🇫🇷

Pessac, France

Uniklinikum "Carl Gustav Carus"; Med. Klinik 1; Hämatologie, Zelltherapie und Medizinische Onkologie

🇩🇪

Dresden, Germany

Institut Paoli Calmettes; Departement D' Onco-Hematologie

🇫🇷

Marseille, France

Istituto Clinico Humanitas;U.O. Oncologia Medica Ed Ematologia

🇮🇹

Rozzano, Lombardia, Italy

National Taiwan Universtiy Hospital; Division of Hematology

🇨🇳

Taipei, Taiwan

ASST PAPA GIOVANNI XXIII; Ematologia

🇮🇹

Bergamo, Lombardia, Italy

Peter MacCallum Cancer Centre; Medical Oncology

🇦🇺

Melbourne, Victoria, Australia

Klinikum der Universität München, Campus Großhadern; Medizinische Klinik und Poliklinik III

🇩🇪

München, Germany

The Alfred

🇦🇺

Melbourne, Victoria, Australia

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