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Anti-Xa Activity of Enoxaparin for Prevention of Venous Thromboembolism in Severe Nephrotic Syndrome.

Phase 2
Terminated
Conditions
Nephrotic Syndrome
Interventions
Registration Number
NCT04558892
Lead Sponsor
Military Institute od Medicine National Research Institute
Brief Summary

The primary objective is to test the hypothesis that enoxaparin efficacy is reduced in severe nephrotic syndrome. Another purpose is to compare two dosing regimens.

Detailed Description

Nephrotic syndrome (NS) is a rare clinical condition characterized by proteinuria exceeding \>3.5 g/24h, hypoalbuminemia, dyslipidemia and edema and is associated with hypercoagulable state. In severe cases, with serum albumin ≤2.5 g/dL, the risk of venous thromboembolic events (VTE) is particularly high, and pharmacological prophylaxis is recommended. However, there is a limited evidence of its efficacy and optimal dosing.

The study is designed as 3 arms clinical trial, with 2 study groups and a single control group. The study groups will include patients with severe NS parallelly, alternately assigned (1:1) into two enoxaparin dosing regimens. The control group will consisted of individuals without proteinuria and edema, similar in terms of age, anthropometric features and renal function to NS patients, who will be administered a standard enoxaparin dose. A peak anti-Xa activity at the steady state will be measured to determine the plasma concentration of enoxaparin. Additional laboratory tests for markers of NS severity, renal function and coagulation system proteins will be performed. The overhydration and body water compartments will be assessed using bioimpedance spectroscopy technique. Nephrotic patients will be followed up by 12 months to assess overt VTE and adverse events associated with enoxaparin use.

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
65
Inclusion Criteria
  • Severe NS, defined as proteinuria exceeding 3.5 g/24h or 50 mg/kg/24h and serum albumin ≤2.5 g/dL;
  • eGFR ≥30 mL/min/1.73 m2.
Exclusion Criteria
  • Body mass index (BMI) ≥40 kg/m2;
  • Low body mass (<45 kg for female, <57 kg for male);
  • Acute VTE;
  • Previously introduced anticoagulation (due to comorbidities);
  • Contraindications for enoxaparin;
  • Pregnancy.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Nephrotic syndrome - fixed dose (NS-FD)EnoxaparinDrug: Enoxaparin; Dose: 40 mg; Administration: once daily subcutaneously.
Nephrotic syndrome - adjusted dose (NS-AD)EnoxaparinDrug: Enoxaparin; Dose: 1 mg/kg of ideal body weight; Administration: once daily subcutaneously.
Control - fixed dose (C-FD)EnoxaparinDrug: Enoxaparin; Dose: 40 mg; Administration: once daily subcutaneously.
Primary Outcome Measures
NameTimeMethod
Therapeutic enoxaparin's anti-Xa activity in nephrotic syndrome.Average: Day 3-5

Anti-Xa activity values ≥ 0.3 IU/mL in the steady state of enoxaparin concentration, on average between days 3 and 5.

Minimum threshold of enoxaparin's anti-Xa activity.Average: Day 3-5

Anti-Xa activity values ≥ 0.2 IU/mL in the steady state of enoxaparin concentration on average between days 3 and 5.

Secondary Outcome Measures
NameTimeMethod
Severity of nephrotic syndrome.Day 0, Day 3-5

Serum or/and urinary concentration of laboratory markers of disease.

Coagulation system protein.Day 0, Day 3-5

Plasma concentration or activity of secondary hemostasis system protein (clotting factors, fibrinolysis factors, regulatory molecules).

Renal function.Day 0, Day 3-5

Estimated glomerular filtration rate (eGFR) calculated with simplified 4-variable Modification of Diet in Renal Disease (MDRD) formula.

Edema.Day 0, Day 3-5

Clinical evaluation of edema using 3-stages scale (I: \<5 kg, II: 5-10 kg, III: \>10 kg) at enrollment and on the day of measuring anti-Xa activity of enoxaparin.

Overhydration.Day 3-5

Overhydration measured by bioimpedance spectroscopy on the day of measuring anti-Xa activity of enoxaparin.

Trial Locations

Locations (1)

Military Institute of Medicine

🇵🇱

Warsaw, Masovian District, Poland

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