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Carbon Dioxide Infusion:Clinical and Histological Appraisal in Chronic Wounds

Completed
Conditions
Wounds
Registration Number
NCT01864967
Lead Sponsor
Institute of Assistance in Plastic Surgery, Sao Paulo
Brief Summary

Carbon dioxide infusion is a non surgical procedure applied via percutaneous, transdermal and subcutaneous. Increase in the concentration of carbon dioxide decreases pH activating local nitric oxide that stimulates collateral vessels formation, vascular endothelial growth factor and basic fibroblast growth factor. Carbon dioxide infusion restores the blood flow in chronic wounds of the lower limbs.

Detailed Description

Ten patients with chronic wounds caused by venous insufficiency at both lower limbs were distributed into groups. Group I was composed by wounds in the right lower limb that received carbon dioxide and group II by wounds in the left lower limb that not received carbon dioxide, both related to the same patient. Carbon dioxide infusion was performed with the needle pointed toward the granulation tissue, 5cm distant from each one at intervals of 4 days. Biopsies were collected from the wounds before carbon dioxide infusion and after the 3rd, 5th and 10th application. Statistical analysis of the data was performed using Wilcoxon's test and Friedman's variance analysis and multiple comparisons test.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
10
Inclusion Criteria
  • chronic wounds caused by venous insufficiency
Exclusion Criteria
  • other diseases of the lower limbs

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
dilatation of peripheral blood vessels, number of capillaries, macrophages and fibroblasts10 sessions (5 weeks) per patient
Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Instute of Assistance in Plastic Surgery of São Paulo

🇧🇷

São Paulo, Brazil

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