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Safety/Feasibility Study of HBOC-201 in Amputation at/Below Knee From Critical Lower Limb Ischemia

Phase 2
Terminated
Conditions
Peripheral Vascular Disease
Interventions
Registration Number
NCT00300040
Lead Sponsor
Biopure Corporation
Brief Summary

The purpose of this study is to assess the safety and feasibility of HBOC-201 in increasing adequate wound healing in patients with severe peripheral vascular disease who are undergoing lower limb amputation.

The hypothesis is that HBOC-201 will pass through the partially occluded lesions in the peripheral arteries in the lower extremity and promote the wound healing process by delivering oxygen to the oxygen deprived tissues. This will reduce the incidence of lower limb wound complications at 60 days post-surgery and may reduce the incidence of a second amputation.

Detailed Description

This is a Phase II, single-blinded, prospectively randomized, parallel-group, placebo controlled study that will evaluate the safety and feasibility of HBOC-201 when administered therapeutically to patients with a peripheral vascular occlusive disorder and who are undergoing lower limb amputation due to critical lower limb ischemia. Subjects will be randomized (1:1) to receive either HBOC-201 or a colloid control (HAES-steril® 6%).

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
16
Inclusion Criteria
  • Subjects ≥ 18 ≤ 75 years of age

  • Scheduled amputation at or above ankle joint, or a more proximal location but below or through the knee joint

  • Documented severe lower extremity peripheral artery occlusive disease confirmed by clinical symptoms of critical limb ischemia and:

    • Frankly gangrenous tissue that merits amputation or
    • Angiographic evidence of occlusive peripheral artery disease within one month of screening
  • Participant or legal representative signs informed consent

  • Willingness to follow study instructions and follow-up visits

Exclusion Criteria
  • No informed consent is obtained
  • If medically inappropriate to administer 250 mL colloidal solution daily for 4 consecutive days
  • Uncontrolled hypertension (BP > 160/90 mm Hg) despite 2 antihypertensive meds or BP > 180/100 mm Hg if untreated
  • Severe liver dysfunction defined by Total Bilirubin > 3 mg/dL or twice the normal limit of serum AST or ALT
  • Symptomatic CVD diagnosed w/ in last 6 months or known high grade carotid stenosis
  • Any severe or unstable medical condition that might interfere w/ the evaluation of study medication
  • Cardiogenic shock (cardiac index < 2 L/min/m2, PCWP > 18 mm Hg)
  • Amputation above knee joint or below ankle joint
  • Any amputation whereby primary skin closure not technically feasible
  • Candidate for percutaneous or surgical revascularization
  • Cardiac failure defined by a NY class III/IV or left ventricular ejection fraction < 30%
  • Life expectancy < 60 days
  • Systemic mastocytosis
  • Previously demonstrated beef product allergy
  • Myocardial infarction w/ in 30 days
  • Participation in another trial w/ in last 30 days
  • Woman who is pregnant or breastfeeding
  • Amputation with known infection at site of skin closure
  • Renal dysfunction requiring dialysis, or serum creatinine level 2.5 mg/dL
  • Peripheral vascular occlusion from cardio arterial emboli
  • Uncontrolled diabetes blood glucose ≥ 400 mg/dL

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
1Hemoglobin glutamer 250 - bovineHemoglobin glutamer 250 - bovine
26% Hydroxyethylstarch6% Hydroxyethylstarch
Primary Outcome Measures
NameTimeMethod
Mortality at 60 days post amputation procedure60(±7 days) post-procedure follow-up visit
Secondary Outcome Measures
NameTimeMethod
30 day follow up mortality; Complete wound healing 30, 60 day follow up; Time to complete wound healing; Re-amputation 60 days; Hospital, ICU & rehab days; TcPO2 change; Quality of Life; Delayed wound healing/complications; Rehospitalization; Surgeries15(±3 days) , 30(±7 days) and 60(±7 days) post-procedure follow-up visits

Trial Locations

Locations (5)

University of Stellenbosch

🇿🇦

Tygerburg, South Africa

Milpark Hospital

🇿🇦

Parktown West, Guateng, South Africa

John Radcliffe Hospital

🇬🇧

Headington, Oxfordshire, United Kingdom

Pretoria Academic Hospital

🇿🇦

Pretoria, Guateng, South Africa

Johannesburg Hospital

🇿🇦

Johannesburg, Guateng, South Africa

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