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Analysis of in Vitro Biological Reaction of Diaphragmatic Prothesis When Congenital Diaphragmatic Hernia (CDH) Repair

Completed
Conditions
Congenital Diaphragmatic Repair
Registration Number
NCT03316924
Lead Sponsor
University Hospital, Strasbourg, France
Brief Summary

In case of CDH, the incomplete development of a part of the diaphragm allows abdominal viscera to herniate into thorax cavity during fetal development and impaired lung and heart development.

After birth, CDH patients who survived thanks to an active reanimation management undergo diaphragmatic repair. In case of large defect, a patch is needed to complete diaphragmatic closure. Thanks to primary cell culture from foreskin, peritoneal epithelium or cremaster dedicated to incineration investigators want to analyse biological integration of actually used prothesis and others innovations.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
1
Inclusion Criteria
  • Minor aged 0 (day of birth) to 14 years
  • Patient for whom an indication of posthectomy and / or cure of inguinal hernia and / or testicular ectopy is retained
  • Patient whose legal representatives have agreed to the use of operational waste from mesenchymal tissues (foreskin, cremaster and peritoneum) destined for incineration.
  • Patient affiliated to a social security scheme
Exclusion Criteria
  • parents don't agree to the use of its operating waste for research purposes

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
cell colonizationsamples are processed immediately after sampling

Study cell colonization

quality of the extracellular matrixsamples are processed immediately after sampling

Quality of the extracellular matrix produced in vitro in contact with the currently used implant materials compared to biomaterials under development

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Les Hôpitaux Universitaires de Strasbourg

🇫🇷

Strasbourg, France

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