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Tongue Dysbiosis Effects on Arterial Pressure of Periodontitis Patients

Phase 4
Conditions
Periodontitis
Normal Blood Pressure
Interventions
Procedure: Periodontal instrumentation
Drug: Placebo mouthwash
Registration Number
NCT04027179
Lead Sponsor
University of Taubate
Brief Summary

Blood pressure control is crucial for individuals' wellbeing. However, many daily aspects such as diet could impair blood pressure control. In addition, many people living under different conditions in different countries are affected by some kind of gum disease. These people experience gingival bleeding, bad breath, teeth mobility and pain. Throughout gum disease development the number of oral germs in the mouth increases including their levels in tongue surface. Oral bacterial are able to convert nitrate widely found in food in nitrite which influences blood pressure. Frequently treatment of gum diseases general combines manual instrumentation with mouthwashes. However, it has been suggested that reduction of oral bacteria by mouthwashes, especially chlorhexidine, is accompanied by decreased conversion of nitrate to nitrite and that this minor nitrite availability would increase blood pressure. Therefore, this is a point to be clarified for patients, physicians and dentists.

This study will investigate the relation between treatment with mouthwashes and blood pressure of patients with destructive gum disease based on nitrite levels in saliva, bacterial levels in tongue and values of arterial blood pressure which will be monitored over 6 months. In addition, usual clinical parameters and alteration of oral cells' DNA will be also monitored overtime. Patients will be treated under local anesthesia and manual instrumentation within 24 hours. They will receive oral care products too. There will be 3 treatment groups (manual instrumentation + chlorhexidine mouthwash \[2 times a day for 3 weeks\], manual instrumentation + placebo mouthwash \[2 times a day for 3 weeks\] and manual instrumentation + no mouthwash) and 2 dental appointments before treatment. After treatment, patients will be examined at 7, 14, 21, 90 and 180 days. Saliva, plaque and cell sampling will be fast and by no invasive methods.

Detailed Description

Not available

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
90
Inclusion Criteria
  • 12 natural teeth; chronic periodontitis stages II and III; no systemic medication; non smokers; normal blood pressure; no mouthwash regular use; antibiotics > 3 months prior to study; dental treatment > 3 months prior to study.
Exclusion Criteria
  • known alergy to chlorhexidine; removable prosthodontics apparatus; pregnant and breast feeding women.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
FMS chlorhexidine mouthwash0.12% chlorhexidine digluconate mouthwashFull-mouth scaling and root planning with manual curettes, 20 ml of 0.12% chlorhexidine gluconate mouthwash irrigation of each periodontal pocket of 5mm of more. Patients will rinse with 0.12% chlorhexidine gluconate mouthwash (20mL/60 seconds/ 2 times a day/ 3 weeks).
FMS chlorhexidine mouthwashPeriodontal instrumentationFull-mouth scaling and root planning with manual curettes, 20 ml of 0.12% chlorhexidine gluconate mouthwash irrigation of each periodontal pocket of 5mm of more. Patients will rinse with 0.12% chlorhexidine gluconate mouthwash (20mL/60 seconds/ 2 times a day/ 3 weeks).
FMS placebo mouthwashPlacebo mouthwashFull-mouth scaling and root planning with manual curettes, 20 ml of placebo mouthwash irrigation of each periodontal pocket of 5mm of more. Patients will rinse with placebo mouthwash (20mL/60 seconds/ 2 times a day/ 3 weeks).
FMS no mouthwashPeriodontal instrumentationFull-mouth scaling and root planning with manual curettes.
FMS placebo mouthwashPeriodontal instrumentationFull-mouth scaling and root planning with manual curettes, 20 ml of placebo mouthwash irrigation of each periodontal pocket of 5mm of more. Patients will rinse with placebo mouthwash (20mL/60 seconds/ 2 times a day/ 3 weeks).
Primary Outcome Measures
NameTimeMethod
Efficacy - comparative tongue bacterial countsBaseline and 3 months

Changes of Veillonella, Streptococcus, Neisseria, Fusobacterium and Acytinomyces counts in samples from tongue dorsal

Secondary Outcome Measures
NameTimeMethod
Safety - comparative nitrite levels in salivaBaseline and 3 months

Differences in mean nitrite levels from saliva samples pre- and post-treatment

Safety - Percentage of hypertension episodesBaseline and 3 months

Changes in the percentage of hypertension episode

Safety - status of DNA methylation in oral cellsBaseline and 3 months

Mean changes of DNA methylation statuses in oral cells

Efficacy - Percentage of periodontal pocketsBaseline and 6 months

Changes in the percentage of deep periodontal pockets

Trial Locations

Locations (1)

University of Taubate - Nucleus of periodontal research

🇧🇷

Taubate, SP, Brazil

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