A higher response of plasma neuropeptide Y, growth hormone, leptin levels and extracellular glycerol levels in subcutaneous abdominal adipose tissue to Acipimox during exercise in patients with bulimia nervosa: microdialysis study

Completed

• Conditions: bulimia nervosa; Mental Health - Eating disorders

• Registration Number: ACTRN12611000955910
• Lead Sponsor: Ministry of Health

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2011-09-06
• Last Update Posted: 13 January 2020

Recruitment & Eligibility

• Status: Completed
• Sex: Female
• Target Recruitment: 16

Inclusion Criteria

age between 18 and 30 years, BMI between 18 and 23 kg/m2, patients with a diagnosis of bulimia nervosa (Diagnostic and Statistical Manual of Mental Disorders, DSM-IV, 1994)

Exclusion Criteria

hypertension, abnormal blood tests with significant hyperlipidaemia, history or presence of hepatic or renal disorders
All eligible participants were deemed healthy.

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
A higher overall response of plasma neuropeptide Y, growth hormone and leptin levels to anti-lipolytic drug Acipimox during the exercise in bulimic patients when compared with healthy women. A blood sample was collected at the beginning and in the course (after 45-minute exercise) of the experiment to estimate plasma neuropeptide Y, growth hormone and leptin concentrations. Blood samples were collected into chilled tubes containing Na2EDTA and antilysin. Plasma was separated immediately by centrifugation and stored until being assayed. Plasma neuropeptide Y concentrations were determined by a commercial radioimmunoassay (Linco Research, St. Charles, Missouri, USA). Plasma growth hormone concentrations were measured by a commercial RIA kit (Immunotech, Prague, Czech Republic). Plasma leptin concentrations were determined by a commercial RIA kit (Linco Research, St. Charles, Missouri, USA).[at one year after randomisation]

Secondary Outcome Measures

Name	Time	Method
A higher local response of extracellular glycerol in subcutaneous abdominal adipose tissue to Acipimox during the exercise in bulimic patients as well as a higher plasma glycerol turnover as the index systemic lipolysis to Acipimox during the exercise in bulimic patients when compared with healthy women. The in situ and in vivo microdialysis technique (CMA Microdialysis, Stockholm, Sweden) was used to examine the exercise-stimulated lipolysis (by measurement of dialysate glycerol). A blood sample was collected at the beginning and in the course (after 45-minute exercise) of the experiment to estimate plasma glycerol concentrations. Blood samples was collected into chilled tubes containing Na2EDTA. Plasma was separated immediatelly by centrifugation and stored until being assayed. Glycerol in the dialysate and in plasma was analyzed with a radiometric kit (Randox Laboratories, GY 105, Montpellier, France).[at one year after randomisation]