Sex Differences in Resistance Exercise-induced Muscle Damage: The Impact of Exercise Load
试验速览
- 阶段
- 不适用
- 状态
- 暂停
- 入组人数
- 40
- 试验地点
- 2
- 主要终点
- Maximal Voluntary Contraction at baseline
研究概览
简要总结
Purpose: To investigate the impact of exercise load on resistance exercise-induced muscle damage in untrained males and females.
Rationale: Unaccustomed resistance exercise can cause muscle damage, presenting as muscle soreness and reduced muscle function - such as loss of strength, power, and flexibility - for several days after the exercise bout. Therefore, individuals may require longer recovery periods before performing another exercise bout, and their performance may be impaired. Further, muscle soreness may reduce exercise compliance, particularly in novice individuals. Over time, this may compromise the gains in muscle mass and strength achieved through exercise training. Therefore, strategies to reduce the severity of exercise-induced muscle damage and/or to enhance post-exercise recovery processes are advantageous for exercising individuals.
One such strategy is to perform resistance exercise with lighter loads, i.e. <70% one repetition maximum (1RM). Low-load resistance training has shown to induce comparable gains in muscle mass and strength to high-load (≥70% 1RM), while being perceptively less exerting. Low-load resistance exercise may place less mechanical stress on muscle fibres and accordingly, its impact on muscle damage has been investigated. While several studies have reported less severe muscle damage, muscle soreness, and functional impairments with low-load resistance exercise compared to high-load, others have found no differences. Further, there is a lack of studies conducted solely in females or comparing between sexes. It has been suggested that males and females respond differently to muscle damage, and therefore, this research aims to provide a sex comparison in the muscle damage response to an acute bout of resistance exercise performed with low or high loads.
Therefore, 40 healthy, young (18-35 years) adults (20 males, 20 females) will be recruited to participate in this randomised controlled trial. Maximal leg strength and body composition (by dual-energy X-ray absorptiometry; DXA) will be conducted at baseline. In females, all primary outcome measures will be obtained during the late follicular phase of the menstrual cycle. Participants will then be randomised to a low-load (30% 1RM) or high-load (80% 1RM) exercise condition.
Three weeks later, participants will complete a resistance exercise session at their allocated intensity on leg extension and leg curl machines to induce muscle damage. Various measures of muscle damage (blood biomarkers, muscle soreness, flexibility, and swelling) will be obtained before, immediately after, and 24, 48, 72, and 168 h after the exercise protocol. The maximal strength test will be repeated 72 and 168 h after the exercise. Participants' habitual activity and dietary intake will be monitored and controlled throughout the study period.
Expected outcome: It is expected that the resistance exercise protocol will induce muscle damage, which will be less severe in the low-load exercise condition. It cannot be ascertained whether males and females will have the same responses to the exercise.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •BMI 18.5 - 25.0 kg/m2
- •Untrained in resistance exercise
- •No known chronic disease or current acute illness
- •No current or recent (past 3 months) musculoskeletal injury
- •No frequent use (2x per week for past month) of non-steroidal anti-inflammatory drugs, anti-oxidant supplements, polyunsaturated omega-3 fatty acids (and other substances that may alleviate muscle damage) and compliant to abstain from use during experimental period
- •No recent or current engagement in massage or cryotherapy and compliant to abstain from use during experimental period
- •Females will be eumenorrheic (regular menstrual cycle) >12 months
- •Absence of pregnancy and breast-feeding
排除标准
- •Underweight
- •Overweight/obese
- •Resistance trained
- •Current or recent injury
- •Pregnancy or breast-feeding
- •Unwilling to provide blood samples, perform resistance exercise, or abstain from use of NSAID's and other substances (stated above)
- •Unwilling to abstain from other forms of exercise during the experimental period
结局指标
主要结局
Maximal Voluntary Contraction at baseline
时间窗: Baseline
One-repetition maximum (1RM) test: leg extension and leg curl machines
Change from baseline Maximal Voluntary Contraction at 72-hours post-exercise
时间窗: 72-hours after the exercise bout
One-repetition maximum (1RM) test: leg extension and leg curl machines
Change from baseline Maximal Voluntary Contraction at 168-hours post-exercise
时间窗: 168-hours after the exercise bout
One-repetition maximum (1RM) test: leg extension and leg curl machines
Change from baseline in Creatine Kinase concentration at 168-hours post-exercise
时间窗: 168-hours after the exercise bout
Serum concentration of creatine kinase from venous blood sampling
Creatine kinase concentration at baseline
时间窗: Immediately pre-exercise
Serum concentration of creatine kinase from venous blood sampling
Change from baseline in Creatine Kinase concentration immediately post-exercise
时间窗: Immediately after the exercise bout
Serum concentration of creatine kinase from venous blood sampling
Change from baseline in Creatine Kinase concentration at 24-hours post-exercise
时间窗: 24-hours after the exercise bout
Serum concentration of creatine kinase from venous blood sampling
Change from baseline in Creatine Kinase concentration at 48-hours post-exercise
时间窗: 48-hours after the exercise bout
Serum concentration of creatine kinase from venous blood sampling
Change from baseline in Creatine Kinase concentration at 72-hours post-exercise
时间窗: 72-hours after the exercise bout
Serum concentration of creatine kinase from venous blood sampling
Interleukin-6 concentration at baseline
时间窗: Immediately pre-exercise
Serum concentration of Interleukin-6 from venous blood sampling
Change from baseline in Interleukin-6 concentration immediately post-exercise
时间窗: Immediately after the exercise bout
Serum concentration of Interleukin-6 from venous blood sampling
Change from baseline in Interleukin-6 concentration at 24-hours post-exercise
时间窗: 24-hours after the exercise bout
Serum concentration of Interleukin-6 from venous blood sampling
Change from baseline in Interleukin-6 concentration at 48-hours post-exercise
时间窗: 48-hours after the exercise bout
Serum concentration of Interleukin-6 from venous blood sampling
Change from baseline in Interleukin-6 concentration at 72-hours post-exercise
时间窗: 72-hours after the exercise bout
Serum concentration of Interleukin-6 from venous blood sampling
Change from baseline in Interleukin-6 concentration at 168-hours post-exercise
时间窗: 168-hours after the exercise bout
Serum concentration of Interleukin-6 from venous blood sampling
Muscle soreness (pressure algometry) at baseline
时间窗: Immediately pre-exercise
Self-perceived rating of muscle soreness with use of pressure algometry
Change in muscle soreness (pressure algometry) immediately post-exercise
时间窗: Immediately after the exercise bout
Self-perceived rating of muscle soreness with use of pressure algometry
Change in muscle soreness (pressure algometry) at 24-hours post-exercise
时间窗: 24-hours after the exercise bout
Self-perceived rating of muscle soreness with use of pressure algometry
Change in muscle soreness (pressure algometry) at 48-hours post-exercise
时间窗: 48-hours after the exercise bout
Self-perceived rating of muscle soreness with use of pressure algometry
Change in muscle soreness (visual analogue scale, VAS) at 24-hours post-exercise
时间窗: 24-hours after the exercise bout
Self-perceived rating of muscle soreness while performing a bodyweight squat with use of a visual analogue scale (0 - not sore at all, 10 - extremely sore)
Change in muscle soreness (visual analogue scale, VAS) at 48-hours post-exercise
时间窗: 48-hours after the exercise bout
Self-perceived rating of muscle soreness while performing a bodyweight squat with use of a visual analogue scale (0 - not sore at all, 10 - extremely sore)
Change in muscle soreness (visual analogue scale, VAS) at 72-hours post-exercise
时间窗: 72-hours after the exercise bout
Self-perceived rating of muscle soreness while performing a bodyweight squat with use of a visual analogue scale (0 - not sore at all, 10 - extremely sore)
Change in muscle soreness (visual analogue scale, VAS) at 168-hours post-exercise
时间窗: 168-hours after the exercise bout
Self-perceived rating of muscle soreness while performing a bodyweight squat with use of a visual analogue scale (0 - not sore at all, 10 - extremely sore)
Range of motion at baseline
时间窗: Immediately pre-exercise
Flexibility of the exercised limb as determined by goniometry
Change in range of motion immediately post-exercise
时间窗: Immediately after the exercise bout
Flexibility of the exercised limb as determined by goniometry
Change in range of motion at 24-hours post-exercise
时间窗: 24-hours after the exercise bout
Flexibility of the exercised limb as determined by goniometry
Change in range of motion at 48-hours post-exercise
时间窗: 48-hours after the exercise bout
Flexibility of the exercised limb as determined by goniometry
Change in range of motion at 72-hours post-exercise
时间窗: 72-hours after the exercise bout
Flexibility of the exercised limb as determined by goniometry
Change in range of motion at 168-hours post-exercise
时间窗: 168-hours after the exercise bout
Flexibility of the exercised limb as determined by goniometry
Limb circumference at baseline
时间窗: Immediately pre-exercise
Measure of leg circumference with use of standard anthropometric tape to indicate muscle swelling
Change in limb circumference immediately post-exercise
时间窗: Immediately after the exercise bout
Measure of leg circumference with use of standard anthropometric tape to indicate muscle swelling
Change in limb circumference at 24-hours post-exercise
时间窗: 24-hours after the exercise bout
Measure of leg circumference with use of standard anthropometric tape to indicate muscle swelling
Change in limb circumference at 48-hours post-exercise
时间窗: 48-hours after the exercise bout
Measure of leg circumference with use of standard anthropometric tape to indicate muscle swelling
Change in limb circumference at 72-hours post-exercise
时间窗: 72-hours after the exercise bout
Measure of leg circumference with use of standard anthropometric tape to indicate muscle swelling
Change in limb circumference at 168-hours post-exercise
时间窗: 168-hours after the exercise bout
Measure of leg circumference with use of standard anthropometric tape to indicate muscle swelling
Change in muscle soreness (pressure algometry) at 72-hours post-exercise
时间窗: 72-hours after the exercise bout
Self-perceived rating of muscle soreness with use of pressure algometry
Change in muscle soreness (pressure algometry) at 168-hours post-exercise
时间窗: 168-hours after the exercise bout
Self-perceived rating of muscle soreness with use of pressure algometry
Muscle soreness (visual analogue scale, VAS) at baseline
时间窗: Immediately pre-exercise
Self-perceived rating of muscle soreness while performing a bodyweight squat with use of a visual analogue scale (0 - not sore at all, 10 - extremely sore)
Change in muscle soreness (visual analogue scale, VAS) immediately post-exercise
时间窗: Immediately after the exercise bout
Self-perceived rating of muscle soreness while performing a bodyweight squat with use of a visual analogue scale (0 - not sore at all, 10 - extremely sore)
次要结局
未报告次要终点
研究者
Alice Pearson
Principal Investigator
Durham University