Study on Antigen-presenting Function of Gamma Delta T Cells in Sepsis and Its Molecular Mechanisms

• Conditions: Sepsis; Antigen-presenting Function; γδ T Cells
• Interventions: Other: Collect peripheral blood sample

• Registration Number: NCT03379896
• Lead Sponsor: West China Hospital

Brief Summary

Study on antigen-presenting function of gamma delta T cells in sepsis and its molecular mechanisms

Detailed Description

The latest definition of sepsis highlights the role of dysregulated host response to infection in organ dysfunction aggravation. Maintenance of immune homeostasis of septic patients through immunotherapy is the key breakthrough to improve success rate. Antigen-presenting cell (APC) is the bridge that connects the innate and adaptive immunity. Decrease of count and function of APC has been shown to be associated with worsened clinical outcomes in patients with sepsis. Gamma-delta T cells (γδ T cells) are the newly identified population of T lymphocytes. Recent studies have found that γδ T cells possess unique and powerful antigen-presenting function, making them the hot topic in infection and cancer research. Based on the results, the investigator plan to evaluate the antigen-presenting function changes of γδ T cells in septic status by analyzing the antigen-presenting related molecules on γδ T cells, antigen protein uptake ability, and their function on the proliferation and activation of CD8+ T lymphocytes. Furthermore, the investigator will explore the mechanism and signal pathways for the APC function changes of γδ T cells. Findings from this research could help explain the mechanism of sepsis induced immune dysfunction, enrich our understanding of the important role of γδ T cells, and build a good foundation for immunotherapy in sepsis.

Study Timeline

• Status Verified: 2017-12
• Study First Submitted: 2017-12-16
• Study First Submitted QC: 2017-12-19
• Study First Posted: 2017-12-20
• Last Update Submitted: 2017-12-20
• Last Update Posted: 2017-12-22
• Study Start: 2018-01-04
• Primary Completion: 2020-06-30
• Study Completion: 2020-12-30

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 60

Inclusion Criteria

1. ≥18 year old;
2. a known or suspected infection based on clinical data at the time of admission and sepsis-induced dysfunction of at least one organ.

Exclusion Criteria

1. autoimmune disease,
2. history of transplantation,
3. acute tuberculosis,
4. chronic hepatitis B or C infection,
5. HIV infection,
6. active malignancy, and
7. long-term use of cortical steroids.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Sepsis	Collect peripheral blood sample	Patient diagnosed with sepsis according to the new sepsis definition (infection+SOFA≥2).
Control	Collect peripheral blood sample	Age-matched healthy control

Primary Outcome Measures

Name	Time	Method
28-day clinical outcome	28-day after ICU admission	death