GnRH analogues for ovarian stimulation and embryo quality: monitoring embryo development using a time lapse incubator.
Completed
- Conditions
- fertility disorderSubfertility10013356
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 82
Inclusion Criteria
Female age <= 37 years
BMI < 32 kg/m2
Regular cycle (25-35 days)
Partner with normal semen parameters (Volume: >= 1.5 ml, concentration: >15*10^6/ml, progressively motile sperm > 32%, so with a VCM >= 7.2)
Standard indication for IVF
Undergoing first or second IVF cycle
Exclusion Criteria
Indication for ICSI
Endometriosis
Expected poor response
One previous IVF treatment not resulting in embryo transfer
Medical contra indication for pregnancy or IVF treatment
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The aim of the study is to analyze the effect of the GnRH analogue used on<br /><br>embryo developmental kinetics. The main study parameter is the time needed from<br /><br>completion of the first cleavage division until reaching the 4-cell stage,<br /><br>determined by retrospective analysis of time lapse recordings. </p><br>
- Secondary Outcome Measures
Name Time Method <p>Secondary study parameters on embryo developmental kinetics will be the time<br /><br>interval between:<br /><br>- Disappearance of the pronuclei and first cleavage of the embryo, as well as<br /><br>reaching the 2-cell, 4-cell and 8-cell stage;<br /><br>- Completion of first cleavage and reaching the 3-cell and 8-cell stage;<br /><br>- The 3-cell and 4-cell stage.<br /><br>We will also analyze embryo morphology on Day 3 and determine the proportion of<br /><br>good quality embryos, as defined by embryos with 8 cells, <20% fragmentation,<br /><br>equally sized blastomeres and no irregularities observed in the cytoplasm. For<br /><br>the embryo that is selected for transfer, the implantation potential will be<br /><br>recorded. </p><br>