Response of Alkylresorcinols to Different Cereals

Not Applicable

Completed

Conditions: Healthy

Interventions: Other: Refined wheat flour
Other: Esterase wholegrain wheat flour
Other: Wholegrain wheat flour
Other: Liquid whole grain wheat flour
Other: Wholegrain barley flour
Other: Liquid wholegrain barley flour

Registration Number: NCT01287403

Lead Sponsor: Société des Produits Nestlé (SPN)

Brief Summary: Cereal grains contain a wide variety of different phytochemicals which may play different roles in physiology. Their response in plasma and urine after a meal is poorly defined for most cereals. This study will investigate the plasma response and urinary excretion of cereal compounds after eating 6 different types of cereal preparation, with a focus on alkylresorcinols.

Detailed Description: This was a cross-over, randomized, double blind study with 6 arms (flours of different composition). Thirteen subjects completed the trial, where 100 g of each flour, mixed with 300 mL milk, was fed to all subjects.

Recruitment & Eligibility

Status: COMPLETED

Sex: All

Target Recruitment: 14

Inclusion Criteria

Healthy
Normal-overweight (BMI 19-28 kg/m2)
Non-smokers

Exclusion Criteria

Regular consumers of wholegrain cereals
Allergy to cereal or milk products

Study & Design

Study Type: INTERVENTIONAL

Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Refined wheat flour	Wholegrain barley flour	A negative control flour to serve as a reference.
Liquid wholegrain barley flour	Refined wheat flour	Test flour with unknown response.
Liquid wholegrain barley flour	Wholegrain wheat flour	Test flour with unknown response.
Esterase wholegrain wheat flour	Wholegrain barley flour	Wholegrain wheat flour treated with esterases to release phenolics (positive control for phenolic acids)
Wholegrain barley flour	Refined wheat flour	Test flour with unknown response.
Refined wheat flour	Esterase wholegrain wheat flour	A negative control flour to serve as a reference.
Refined wheat flour	Wholegrain wheat flour	A negative control flour to serve as a reference.
Refined wheat flour	Liquid whole grain wheat flour	A negative control flour to serve as a reference.
Esterase wholegrain wheat flour	Wholegrain wheat flour	Wholegrain wheat flour treated with esterases to release phenolics (positive control for phenolic acids)
Wholegrain wheat flour	Liquid whole grain wheat flour	Flour with unknown response
Liquid whole grain wheat flour	Esterase wholegrain wheat flour	Test flour with unknown response.
Wholegrain barley flour	Esterase wholegrain wheat flour	Test flour with unknown response.
Wholegrain barley flour	Wholegrain wheat flour	Test flour with unknown response.
Liquid wholegrain barley flour	Esterase wholegrain wheat flour	Test flour with unknown response.
Refined wheat flour	Liquid wholegrain barley flour	A negative control flour to serve as a reference.
Esterase wholegrain wheat flour	Liquid whole grain wheat flour	Wholegrain wheat flour treated with esterases to release phenolics (positive control for phenolic acids)
Wholegrain wheat flour	Refined wheat flour	Flour with unknown response
Wholegrain wheat flour	Wholegrain barley flour	Flour with unknown response
Wholegrain wheat flour	Liquid wholegrain barley flour	Flour with unknown response
Liquid whole grain wheat flour	Liquid wholegrain barley flour	Test flour with unknown response.
Liquid wholegrain barley flour	Liquid whole grain wheat flour	Test flour with unknown response.
Liquid wholegrain barley flour	Wholegrain barley flour	Test flour with unknown response.
Esterase wholegrain wheat flour	Refined wheat flour	Wholegrain wheat flour treated with esterases to release phenolics (positive control for phenolic acids)
Esterase wholegrain wheat flour	Liquid wholegrain barley flour	Wholegrain wheat flour treated with esterases to release phenolics (positive control for phenolic acids)
Wholegrain wheat flour	Esterase wholegrain wheat flour	Flour with unknown response
Liquid whole grain wheat flour	Refined wheat flour	Test flour with unknown response.
Liquid whole grain wheat flour	Wholegrain wheat flour	Test flour with unknown response.
Liquid whole grain wheat flour	Wholegrain barley flour	Test flour with unknown response.
Wholegrain barley flour	Liquid whole grain wheat flour	Test flour with unknown response.
Wholegrain barley flour	Liquid wholegrain barley flour	Test flour with unknown response.

Primary Outcome Measures

Name	Time	Method
Plasma Alkylresorcinol Compounds	From 0 to 48 h post dose.	Area under the curve (AUC) over baseline of alkylresorcinol compounds are measured for the 6 interventional arms. Time points are: t = 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 14, 24 and 48 h after product intake.

Secondary Outcome Measures