Effects of Exercise on Fructose Metabolism
- Conditions
- Healthy Subjects
- Interventions
- Other: exerciseDietary Supplement: fructoseDietary Supplement: glucose
- Registration Number
- NCT01866215
- Lead Sponsor
- University of Lausanne
- Brief Summary
A high fructose diet increases fasting and post-prandial triglyceride (TG) concentrations in sedentary healthy human subjects.These effects may be secondary to fructose-induced hepatic de novo lipogenesis. Recent evidence indicate that exercise can prevent fructose induced dyslipidemia.This study will evaluate
1. how exercise effects the metabolic fate of oral fructose 1a) when exercise is performed before fructose ingestion 1b) when exercise is performed after fructose ingestion Metabolic effects of exercise will be assessed in healthy male subjects by measuring fructose oxidation (13CO2 production), fructose conversion into glucose (13C glucose concentrations in blood) and hepatic fructose conversion into lipid (13C palmitate-very low density lipoprotein (VLDL) concentrations in blood) after ingestion of 13C-labelled fructose meals
2. how fructose and protein modulate muscle glycogen and intramyocellular lipid repletion after exercise Healthy male subjects will be fed various fructose, glucose, lipid and whey protein meals after a glycogen/intramyocellular lipid depleting exercise. The effects of meals' composition will be assessed after 24 hours by measuring intramyocellular lipids and glycogen using proton-magnetic resonance spectroscopy (MRS).
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 8
- males
- 18-40 years old
- 19 kg/m2>BMI>25kg/m2
- moderate to high usual physical activity
- non-smokers
- family history of diabetes mellitus
- ECG anomaly
- any medication
- participation to blood spending or other clinical study in the 3 months before the beginning of this study
- consumption of drugs
- consumption of more than 50g alcool/week
- family history of food intolerance
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Study1a exercise exercice performed 90 min before 13C fructose meal ingestion study 1b exercise exercise performed 90 min after 13C fructose meal ingestion study 2a fructose meals containing fructose, cream and whey proteins over 24 hours after a glycogen/intramyocellular lipid depleting exercise study 2b glucose meals containing glucose, cream and whey proteins over 24 hours after a glycogen/intramyocellular lipid depleting exercise
- Primary Outcome Measures
Name Time Method Plasma triglyceride measurements in fasting conditions and every hour from 7:00 am to 4:00 pm (up to 9 hours) measurement of total and VLDL-TG concentrations in study 1
intramyocellular lipids after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise Measurement by 1H-MRS in muscle vastus lateralis in study 2
Fructose conversion into lipids measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours) Estimated from 13C palmitate-VLDL concentration
- Secondary Outcome Measures
Name Time Method Fructose conversion into glucose measured between 7:00 am and 4:oo pm (up to 9 hours) measurements obtained from plasma 13C glucose concentrations and whole body glucose production measured by 6,6 d2 glucose in study 1)
intramyocellular glycogen concentrations after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise Measured by 1H-MRS in muscle vastus lateralis in study 2
fructose oxidation measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours) Calculated from breath 13CO2 production
exogenous lipid oxidation measured from 7:00 am to 4:00 pm in study 2 (up to 9 hours) Lipids ingested will be labeled with 13C-triolein, and exogenous lipid oxidation will be measured from breath 13CO2 production
Trial Locations
- Locations (1)
Centre Hospitalier Universitaire Vaudois
🇨ðŸ‡Lausanne, Vaud, Switzerland