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Post COVID-19 Syndrome and the Gut-lung Axis

Not Applicable
Active, not recruiting
Conditions
Covid19
Interventions
Dietary Supplement: Placebo
Dietary Supplement: Omni-Biotic Pro Vi 5
Registration Number
NCT04813718
Lead Sponsor
Medical University of Graz
Brief Summary

The outbreak of the novel coronavirus (SARS-CoV-2)-infected disease (COVID-19) began in December 2019, spread throughout China in early 2020 and developed as a pandemic thereafter.

Based on current knowledge, Covid-19 infection causes mild to moderate respiratory and gastrointestinal symptoms in the majority of patients. In a smaller percentage severe disease courses are observed, often with the need of hospitalization and intensive care treatment. Apparently, symptoms can persist for relatively long time after viral clearance, suggesting the existence of a "Post-Covid" syndrome. A study from the UK identified fatigue, breathlessness and psychosocial stress as common symptoms after discharge from the hospital. Covid-19 infection is frequently characterized by a hyperinflammatory phenotype and a cytokine storm. The Covid-19 cytokine storm is characterised by rapid proliferation and hyperactivation of T cells, macrophages, mast cells, neutrophil granulocytes and natural killer cells, and the overproduction of inflammatory cytokines and chemical mediators released by immune or nonimmune cells. Early data also suggest that even if symptoms are just 'mild to moderate' during the acute infection, fibrotic lung damage develops in some patients. This may lead to long-term pulmonary complications for a subset of patients. The mechanisms for post-Covid pulmonary fibrosis are still unclear: inflammation triggering fibrosis, epithelial and endothelial injury with inadequate fibroproliferation and vascular damage are considered to be possible mechanisms.

A potential therapeutic target in ameliorating post-Covid symptoms could be the gut microbiome. Gut microbiome alterations have been described in Covid-19. The gut-lung axis as a link between dysbiosis, barrier dysfunction, translocation of bacterial products and hyperinflammation has been proposed as a potential therapeutic target. Probiotics have been proposed to be a possible modulator of the deranged gut-lung axis in Covid-disease and post-Covid syndrome. Currently 11 studies are registered in clinicaltrials.gov for treatment of acute Covid disease and prevention of the disease (including one study from Graz), but no study related to post-Covid syndrome could be found.

Therefore, it is currently unclear, which clinical, immune system or microbiome related biomarker would be the best to study the effect of a microbiome-based intervention in post-Covid syndrome.

Detailed Description

Not available

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
All
Target Recruitment
20
Inclusion Criteria
  • 18 years or older
  • Covid-19 infection with severe disease defined within the last 12 months (defined as one or more of the following: hospitalization, need for oxygen supply, need for intensive care treatment, need for specific treatment of Covid disease, antibiotic treatment)
  • Subjective presence of residual symptoms of Covid disease OR no residual symptoms of Covid disease (Controls)
  • Informed consent
Exclusion Criteria
  • Continuous probiotic treatment in the last 4 weeks before inclusion
  • Pre-existing lung diseases

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboPlacebosimilar looking and tasting
SynbioticOmni-Biotic Pro Vi 5Omni-Biotic Pro Vi 5
Primary Outcome Measures
NameTimeMethod
TNFalpha6 months

Change in TNFalpha levels serum over time and with/without the intervention

B cells immunophenotyping6 months

Change in B cell subtypes over time and with/without the intervention

Interleukin 66 months

Change in interleukin 6 serum levels over time and with/without the intervention

Interleukin 6 receptor6 months

Change in Interleukin 6 receptor serum levels over time and with/without the intervention

interleukin 86 months

Change in interleukin 8 serum levels over time and with/without the intervention

interleukin 106 months

Change in interleukin 10 serum levels over time and with/without the intervention

monocyte phagocytosis6 months

Change in monocyte phagocytosis over time and with/without the intervention

Intestinal barrier6 months

Change in zonulin levels over time and with/without the intervention

endotoxin6 months

Change in endotoxin levels serum over time and with/without the intervention

monocyte function surface receptor expression6 months

Change in monocyte surface receptor expression over time and with/without the intervention

T cell immunophenotyping6 months

Change in T cell subtypes over time and with/without the intervention

sCD146 months

Change in sCD14 levels over time and with/without the intervention

interleukin 236 months

Change in interleukin 23 serum levels over time and with/without the intervention

neutrophil function burst function6 months

Change in neutrophil burst function over time and with/without the intervention

neutrophil function phagocytosis6 months

Change in neutrophil phagocytosis function over time and with/without the intervention

Microbiome composition6 months

16 sRNA sequencing

Interleukin 1b6 months

Change in Interleukin 1b serum levels over time and with/without the intervention

interleukin 176 months

Change in interleukin 17 serum levels over time and with/without the intervention

Spirometry6 months

Change in spirometry measurements over time and with/without the intervention

Gas diffusion6 months

Change in pulmonary gas diffusion over time and with/without the intervention

neutrophil NET formation6 months

Change in neutrophil NET formation over time and with/without the intervention

neutrophil surface receptor expression6 months

Change in neutrophil surface receptor expression over time and with/without the intervention

monocyte burst6 months

Change in monocyte burst over time and with/without the intervention

Lung volume6 months

Change in lung volume over time and with/without the intervention

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Medical University Graz

🇦🇹

Graz, Austria

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